UNALTERED NEURONAL AND GLIAL COUNTS IN ANIMAL MODELS OF MAGNETIC SEIZURE THERAPY AND ELECTROCONVULSIVE THERAPY
UNALTERED NEURONAL AND GLIAL COUNTS IN ANIMAL MODELS OF MAGNETIC SEIZURE THERAPY AND ELECTROCONVULSIVE THERAPY
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DOI：
10.1016/j.neuroscience.2009.09.051
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发表时间：
2009-12-29
期刊：
影响因子：
3.3
通讯作者：
Lisanby, S. H.
中科院分区：
文献类型：
--
作者：
Dwork, A. J.;Christensen, J. R.;Lisanby, S. H.
电休克治疗（ECT）对脑损伤的解剖学证据缺乏;但没有现代灵长类动物体视学研究证明其安全性。磁惊厥治疗（MST）是一种创伤性较小的惊厥治疗方法，目前只有一份关于其解剖学效应的报告。我们发现，在模拟人类使用的条件下，高等哺乳动物遭受电休克（ECS）或MST的大脑中没有组织学病变。我们试图通过确定这些干预措施是否会影响额叶皮层或海马中的神经元或神经胶质的数量来扩展这些发现。24只动物接受为期6周的ECS、MST或单独麻醉，每周4天。灌流固定后，用无偏体视学方法测定额叶皮质和海马神经元和胶质细胞的数量。我们发现，无论是干预海马，额叶皮层，或这些结构的子区域的神经元或胶质细胞的体积或总数或数密度没有影响。在人类ECT和MST治疗的严格模型中诱导癫痫发作不会引起大脑潜在脆弱区域中神经元或神经胶质细胞数量的变化。这项研究虽然仅限于年轻、健康的成年受试者，但提供了进一步的证据，证明ECT和MST在适当应用时不会对大脑造成结构性损伤。(C)2009年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Anatomical evidence of brain damage from electroconvulsive therapy (ECT) is lacking; but there are no modern stereological studies in primates documenting its safety. Magnetic seizure therapy (MST) is under development as a less invasive form of convulsive therapy, and there is only one prior report on its anatomical effects. We discerned no histological lesions in the brains of higher mammals subjected to electroconvulsive shock (ECS) or MST, under conditions that model closely those used in humans. We sought to extend these findings by determining whether these interventions affected the number of neurons or glia in the frontal cortex or hippocampus. Twenty-four animals received 6 weeks of ECS, MST, or anesthesia alone, 4 days per week. After perfusion fixation, numbers of neurons and glia in frontal cortex and hippocampus were determined by unbiased stereological methods. We found no effect of either intervention on volumes or total number or numerical density of neurons or glia in hippocampus, frontal cortex, or subregions of these structures. Induction of seizures in a rigorous model of human ECT and MST therapy does not cause a change in the number of neurons or glia in potentially vulnerable regions of brain. This study, while limited to young, healthy, adult subjects, provides further evidence that ECT and MST, when appropriately applied, do not cause structural damage to the brain. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.