A SPECTROPHOMETRIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF MYOGLOBIN AND HEMOGLOBIN IN EXTRACTS OF HUMAN MUSCLE
A SPECTROPHOMETRIC METHOD FOR THE SIMULTANEOUS DETERMINATION OF MYOGLOBIN AND HEMOGLOBIN IN EXTRACTS OF HUMAN MUSCLE
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DOI：
10.3891/acta.chem.scand.02-0264
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发表时间：
1948-01-01
期刊：
影响因子：
--
通讯作者：
DEDUVE, C
中科院分区：
文献类型：
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作者：
DEDUVE, C
使用贝克曼分光光度计可以高精度地对两种色素的混合物中的人类 Hb 和 Mgb 进行差异检测。 Hb和Mgb的CO化合物在600至520mμ之间以及由这些物质形成的还原吡啶血色原的准确分光光度常数是该方法的基础。描述了一种从人类心脏和骨骼肌组织中获得合适提取物的方法。
The differential detn of human Hb and Mgb in a mixture of both pigments can be made with good accuracy, using the Beckman spectrophotometer. Accurate spectrophotometric constants for the CO compounds of Hb and Mgb between 600 and 520 m[mu] and for the reduced pyridine hemochromogens formed from these substances are the basis of the method. A method for obtaining suitable extracts from human heart and skeletal muscle-tissue is described.