Time-Course Transcriptome Study Reveals Mode of bZIP Transcription Factors on Light Exposure in Arabidopsis
Time-Course Transcriptome Study Reveals Mode of bZIP Transcription Factors on Light Exposure in Arabidopsis
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DOI：
10.3390/ijms21061993
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发表时间：
2020-03-01
影响因子：
5.6
通讯作者：
Matsui, Minami
中科院分区：
文献类型：
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作者：
Kurihara, Yukio;Makita, Yuko;Matsui, Minami
萌发后发生的黄化过程，一旦感觉到光线，就会终止，然后开始去黄化。在去黄化期间，单色光(蓝光、红光和远红光)通过各自的光受体诱导基因表达谱和植物行为的差异。延长的下胚轴5(HY5)是一种bZIP型转录因子，在去黄化过程中调节基因的表达，其他bZIP转录因子也参与了这一调节。然而，黄化幼苗在单色光照射下发生的转录变化及其与bZIP转录因子的关系仍有待阐明。在这里，我们跟踪了黑暗后暴露在白色、蓝色、红色和远红光下的转录组的变化，并揭示了这四种光之间转录组变化的共同和非共同趋势。有趣的是，在光照后，HY5的表达与相关的bZIP TF基因GBF2和GBF3同步，而不是HY5同源基因(HYH)。为了推测bZIP转录因子之间的靶基因冗余，我们用体外结合试验检测了7个bZIP转录因子HY5、HYH、GBF1、GBF2、GBF3、GBF4和鳗鱼的同源二聚体的全基因组物理结合位点。结果显示目标基因候选有很大的重叠，这表明在转录因子之间存在复杂的调控文献。这项工作为理解植物对单色光照射反应的基因表达调控提供了新的见解。
The etiolation process, which occurs after germination, is terminated once light is perceived and then de-etiolation commences. During the de-etiolation period, monochromatic lights (blue, red and far-red) induce differences in gene expression profiles and plant behavior through their respective photoreceptors. ELONGATED HYPOCOTYL 5 (HY5), a bZIP-type transcription factor (TF), regulates gene expression in the de-etiolation process, and other bZIP TFs are also involved in this regulation. However, transcriptomic changes that occur in etiolated seedlings upon monochromatic light irradiation and the relationship with the bZIP TFs still remain to be elucidated. Here, we track changes in the transcriptome after exposure to white, blue, red and far-red light following darkness and reveal both shared and non-shared trends of transcriptomic change between the four kinds of light. Interestingly, after exposure to light, HY5 expression synchronized with those of the related bZIP TF genes, GBF2 and GBF3, rather than HY5 HOMOLOG (HYH). To speculate on the redundancy of target genes between the bZIP TFs, we inspected the genome-wide physical binding sites of homodimers of seven bZIP TFs, HY5, HYH, GBF1, GBF2, GBF3, GBF4 and EEL, using an in vitro binding assay. The results reveal large overlaps of target gene candidates, indicating a complicated regulatory literature among TFs. This work provides novel insight into understanding the regulation of gene expression of the plant response to monochromatic light irradiation.