Muricauda nanhaiensis sp. nov., isolated from seawater of the South China Sea
Muricauda nanhaiensis sp. nov., isolated from seawater of the South China Sea
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南海鼠尾鱼
DOI：
10.1099/ijsem.0.003437
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发表时间：
2019-07-01
影响因子：
2.8
通讯作者：
Zhang, Xi-Ying
中科院分区：
文献类型：
--
作者：
Dang, Yan-Ru;Sun, Yuan-Yuan;Zhang, Xi-Ying
从南中国海区表层海水中分离到一株革兰氏染色阴性、需氧、氧化酶和过氧化氢酶阳性、无鞭毛、不滑行、黄色素、杆状附属物的细菌，命名为SM1704(T)。该菌株在15-42摄氏度和1-10%的氯化钠环境中生长。它能降解秦皮甲素，但不能分解明胶和吐温80，也不能将硝酸盐还原为亚硝酸盐。基于16S rRNA基因序列的系统发育分析表明，SM1704(T)菌株属于小鼠尾目属，与已识别种的模式菌株有94.1-95.9%的序列相似性。主要脂肪酸为iso-C-15：0、iso-C-15：1G和iso-C-17：03-OH，主要极性脂类为磷脂酰乙醇胺、3种未知脂和3种未知氨基脂。呼吸性苯二酚主要为孟喹酮-6。菌株SM1704(T)的基因组DNA G+C含量为40.7mol%。根据菌株SM1704(T)的多相分析结果，认为它代表了小尾目昆虫属的一个新种，命名为南海小尾藻Muricauda nanhaiensis sp.11月是提议的。模式菌株为SM1704(T)(=KCTC 62797(T)=MCCC1K03557(T))。
A Gram-stain-negative, aerobic, oxidase- and catalase-positive, non-flagellated, non-gliding, yellow-pigmented, and rod-shaped bacterium with appendages, designated strain SM1704(T), was isolated from surface seawater collected from the South China Sea. The strain grew at 15-42 degrees C and with 1-10 % NaCl. It hydrolysed aesculin, but did not hydrolyse gelatin and Tween 80 nor reduce nitrate to nitrite. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain SM1704(T) was affiliated with the genus Muricauda, sharing 94.1-95.9 % sequence similarities with type strains of recognized Muricauda species. The major fatty acids were iso-C-15:0, iso-C-15:1 G and iso-C-17:0 3-OH and the main polar lipids were phosphatidylethanolamine, three unidentified lipids and three unidentified aminolipids. The major respiratory quinone was menaquinone-6. The genomic DNA G+C content of strain SM1704(T) was 40.7 mol%. On the basis of results from polyphasic analysis of strain SM1704(T), it is considered to represent a novel species within the genus Muricauda, for which the name Muricauda nanhaiensis sp. nov. is proposed. The type strain is SM1704(T) (=KCTC 62797(T)=MCCC 1 K03557(T)).