A quantitative method for the high throughput screening for the soil adhesion properties of plant and microbial polysaccharides and exudates
A quantitative method for the high throughput screening for the soil adhesion properties of plant and microbial polysaccharides and exudates
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DOI：
10.1007/s11104-018-3670-1
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发表时间：
2018-07-01
期刊：
影响因子：
4.9
通讯作者：
Knox, Paul
中科院分区：
文献类型：
--
作者：
Akhtar, Jumana;Galloway, Andrew F.;Knox, Paul
了解土壤中碳基分子的结构和功能是土壤作为一种极其重要的生态系统功能的一个重要目标。多糖与维持土壤团聚体状态有关，但其结构和土壤粘附特性尚未得到广泛的研究。这在很大程度上是因为在确定多糖结构和量化任何功能properties.在这里，我们描述了使用一种新的硝化纤维素为基础的粘附测定，以确定土壤粘附的相对能力超过20植物和微生物多糖，可能存在于土壤中，并有助于有机质含量和性能的技术困难。通过扫描硝化纤维素片，对附着在已知量的特定多糖点上的土壤重量进行定量。从这次调查中确定的最有效的多糖包括壳聚糖、β-1，3-葡聚糖、黄蓍胶、黄原胶和木葡聚糖。我们还表明，土壤粘附性测定是合适的，以评估土壤的植物分泌物的绑定属性。土壤粘附性测定将是有用的功能解剖的土壤有机质组分，也参与土壤附着到植物根系和根鞘形成的因素。
Understanding the structures and functions of carbon-based molecules in soils is an important goal in the context of soils as an ecosystem function of immense importance. Polysaccharides are implicated in maintaining soil aggregate status but have not been extensively dissected in terms of their structures and soil adhesion properties. This is largely because of the technical difficulties in identifying polysaccharide structures and quantifying any functional properties.Here, we describe the use of a novel nitrocellulose-based adhesion assay to determine the relative capacities for soil adhesion of over twenty plant and microbial polysaccharides that are likely to be present in soil and to contribute to organic matter content and properties. Weights of soil adhered to spots of known amounts of specific polysaccharides were quantified by scanning of the nitrocellulose sheets.The most effective polysaccharides identified from this survey included chitosan, beta-1,3-glucan, gum tragacanth, xanthan and xyloglucan. We also demonstrate that the soil adhesion assay is suitable to assess the soil-binding properties of plant exudates.The soil adhesion assay will be useful for the functional dissection of the organic matter components of soils and also of the factors involved in soil attachment to plant roots and in rhizosheath formation.