Isolation of a full-length cDNA encoding mouse aromatase P450.
Isolation of a full-length cDNA encoding mouse aromatase P450.
复制标题
编码小鼠芳香酶 P450 的全长 cDNA 的分离。
DOI:
10.1016/0003-9861(91)90354-l
复制
发表时间:
1991
影响因子:
3.9
通讯作者:
Yutaka Shizuta
中科院分区:
文献类型:
--
作者:
M. Terashima;K. Toda;T. Kawamoto;I. Kuribayashi;Y. Ogawa;T. Maeda;Yutaka Shizuta
A full-length cDNA clone for aromatase P450 has been isolated from a pregnant mouse ovarian cDNA library. The insert of this clone (2394 bp) contains a 1509-bp open reading frame encoding 503 amino acid residues together with a 46-bp 5′-untranslated stretch and an 839-bp 3′-untranslated region to which a poly(A) tract is attached. Northern blot analysis of ovarian RNA from pregnant mice reveals a major mRNA band of 2.5 kb with a minor band of 2.1 kb. Comparison of mouse aromatase P450 with that of rat, human, and chicken shows 91, 81, and 69% identity in the nucleotide sequence and 92, 79, and 69% identity in the deduced amino acid sequence, respectively. The membrane-spanning domain of mouse aromatase P450 is estimated to be an extremely hydrophobic segment located within the N-terminal region of the molecule. Furthermore, a highly conserved heme-binding domain is noticed.
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DOI:
--
发表时间:
1988
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
McPhaul,MJ;Noble,JF;Simpson,ER;Mendelson,CR;Wilson,JD
通讯作者:
Wilson,JD
影响因子:
--
作者:
HICKEY, GJ;KRASNOW, JS;RICHARDS, JS
通讯作者:
RICHARDS, JS
DOI:
--
发表时间:
1987
期刊:
The Journal of biological chemistry
影响因子:
--
作者:
Evans,CT;Corbin,CJ;Saunders,CT;Merrill,JC;Simpson,ER;Mendelson,CR
通讯作者:
Mendelson,CR
影响因子:
4.8
作者:
Lanoux,MJ;Cleland,WH;Mendelson,CR;Carr,BR;Simpson,ER
通讯作者:
Simpson,ER
影响因子:
4.8
作者:
Mendelson,CR;Corbin,CJ;Smith,ME;Smith,J;Simpson,ER
通讯作者:
Simpson,ER