Gut bacteria facilitate adaptation to crop rotation in the western corn rootworm
Gut bacteria facilitate adaptation to crop rotation in the western corn rootworm
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DOI：
10.1073/pnas.1301886110
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发表时间：
2013-07-16
影响因子：
11.1
通讯作者：
Seufferheld, Manfredo J.
中科院分区：
文献类型：
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作者：
Chu, Chia-Ching;Spencer, Joseph L.;Seufferheld, Manfredo J.
昆虫不断适应人类驱动的景观变化；然而，他们的肠道微生物群在这些过程中的作用在很大程度上仍然未知。西部玉米根虫（WCR, Diabrotica virgifera virgifera LeConte）（鞘翅目：金曲蝇科）是一种主要的玉米害虫，在美国通过玉米（Zea mays）和非寄主大豆（Glycine max）之间的年度轮作来控制。这种做法选择了一种“抗旋转”变异（RR-WCR），其对玉米地的产卵保真度降低。在大豆田间，RR-WCRs对大豆叶片中表达的抗草食防御（即半胱氨酸蛋白酶抑制剂）也表现出更高的耐受性。本研究表明，肠道细菌微生物群是促进玉米专家（WCR）对大豆短暂食草性生理适应的重要因素。野生型WCR和野生型WCR的肠道菌群比较显示，细菌群落结构随着宿主对大豆日粮的适应而发生变化。抗生素抑制肠道细菌显著降低大豆草食对RR-WCR的耐受性至WT-WCR水平，而WT-WCR不受影响。我们的研究结果表明，肠道细菌有助于促进昆虫在管理生态系统中的快速适应。
Insects are constantly adapting to human-driven landscape changes; however, the roles of their gut microbiota in these processes remain largely unknown. The western corn rootworm (WCR, Diabrotica virgifera virgifera LeConte) (Coleoptera: Chrysomelidae) is a major corn pest that has been controlled via annual rotation between corn (Zea mays) and nonhost soybean (Glycine max) in the United States. This practice selected for a "rotation-resistant" variant (RR-WCR) with reduced ovipositional fidelity to cornfields. When in soybean fields, RR-WCRs also exhibit an elevated tolerance of antiherbivory defenses (i.e., cysteine protease inhibitors) expressed in soybean foliage. Here we show that gut bacterial microbiota is an important factor facilitating this corn specialist's (WCR's) physiological adaptation to brief soybean herbivory. Comparisons of gut microbiota between RR-and wild-type WCR (WT-WCR) revealed concomitant shifts in bacterial community structure with host adaptation to soybean diets. Antibiotic suppression of gut bacteria significantly reduced RR-WCR tolerance of soybean herbivory to the level of WT-WCR, whereas WT-WCR were unaffected. Our findings demonstrate that gut bacteria help to facilitate rapid adaptation of insects inmanaged ecosystems.