Genotypic characterization and comparison of Streptococcus mutans in American Indian and Southeast Iowa children.
Genotypic characterization and comparison of Streptococcus mutans in American Indian and Southeast Iowa children.
复制标题
美洲印第安人和爱荷华州东南部儿童中变形链球菌的基因型特征和比较。
DOI：
10.1002/cre2.94
复制
发表时间：
2017
影响因子：
1.8
通讯作者：
Drake,DavidR
中科院分区：
文献类型：
--
作者：
Villhauer,AlissaL;Lynch,DavidJ;Warren,JohnJ;Dawson,DeborahV;Blanchette,DerekR;Drake,DavidR
幼儿龋齿是一个复杂的卫生保健问题，有一个多因素的病因。这种病因学的一个方面是产酸细菌在早期的定植和繁殖。有几种细菌与龋齿有关，但一个常见的物种是变形链球菌。在这里，我们描述了从两组具有相似社会经济人口统计学特征的儿童中回收的变异链球菌的基因型多样性和共性：北方平原美国印第安部落和东南部爱荷华州人口。从239名母亲和儿童的队列中选择了40名36个月大的美国印第安儿童，并从东南部爱荷华州选择了40名2至5岁的儿童，以比较从每个儿童的斑块中回收的变形链球菌的基因型谱。从整个口腔菌斑样本中选择突变分离物;提取DNA并通过AP-PCR进行扩增，以显示特定的基因型模式。用GelComparIIv6.5凝胶分析软件对这些图谱进行比较。我们发现18个不同的基因型从524株，其中13个是共同的两个社区。五种基因型仅在美国印第安儿童中存在，而东南部爱荷华州儿童中没有独特的基因型。虽然美国印第安儿童有一些基因型，不存在于东南爱荷华州的儿童，这些并没有广泛分布在社区。此外，两个群体之间的基因型多样性和共同性水平相似。这项研究奠定了基础的基因型和龋齿之间的两个人口的较大的子部分进行全面比较。
Early childhood caries is a complex health care issue that has a multifactorial etiology. One aspect of this etiology is the colonization and propagation of acidogenic bacteria at an early age. There have been several bacterial species associated with caries but 1 common species isStreptococcus mutans. Here, we describe genotypic diversity and commonality ofStreptococcus mutansrecovered from children representing 2 groups with similar socioeconomic demographics: a Northern Plains American Indian Tribe and a Southeast Iowa population. Forty 36‐month‐old American Indian children were selected from a cohort of 239 mothers and children, and forty 2‐ to 5‐year‐old children from Southeast Iowa were selected to compare the genotypic profiles ofStreptococcus mutansrecovered from each child's plaque.S. mutansisolates were selected from whole mouth plaque samples; DNA was extracted and amplified via AP‐PCR to show specific genotype patterns. These patterns were compared with GelComparIIv6.5 gel analysis software. We found 18 distinct genotypes from 524 isolates; 13 of which were common between the 2 communities. Five genotypes were unique to only the American Indian children while the Southeast Iowa children harbored no unique genotypes. Although the American Indian children had some genotypes that were not present in the Southeast Iowa children, these were not widely distributed among the community. Furthermore, the levels of genotypic diversity and commonality were similar between the 2 populations. This study sets the groundwork for a comprehensive comparison of genotypes and caries among larger subsections of both populations.