Glycoconjugates secreted by bovine tracheal serous cells in culture.
Glycoconjugates secreted by bovine tracheal serous cells in culture.
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培养物中牛气管浆液细胞分泌的糖缀合物。
DOI：
10.1016/0003-9861(88)90426-2
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发表时间：
1988
影响因子：
3.9
通讯作者：
Basbaum,CB
中科院分区：
文献类型：
--
作者：
Paul,A;Picard,J;Mergey,M;Veissiere,D;Finkbeiner,WE;Basbaum,CB
牛气管腺浆液性细胞分泌的糖缀合物的特点后，纳入放射性前体[1- 14 C]葡萄糖胺和刺激异丙肾上腺素。在解离条件下，糖缀合物在Sepharose Cl-4 B上的空隙和包含体积中洗脱。通过阴离子交换色谱分离，高分子量（Sepharose Cl-4 B; V0）糖缀合物得到两个酸性馏分，在0.5和2.0mNaCl洗脱;低分子量糖缀合物的包括体积得到一个中性馏分和两个酸性馏分，在0.5和2.0mNaCl洗脱。基于化学分析和特定的酶消化，显示空隙体积中洗脱的材料含有透明质酸和硫酸软骨素蛋白聚糖。此外，少量半乳糖、岩藻糖、唾液酸、葡糖胺和半乳糖胺的存在表明空隙体积中存在O-糖苷连接的糖蛋白。该材料中β-消除寡糖中半乳糖胺醇的鉴别证实了这一观点。在所含体积中洗脱的材料显示含有N-连接糖蛋白，中性组分中含有复合型聚糖，两种酸性组分中含有硫酸软骨素蛋白聚糖。在0.5macalcohol级分中检测到氨基糖的显著N-硫酸化，表明硫酸乙酰肝素的存在。最近已确定在气管分泌物中的透明质酸和硫酸软骨素蛋白聚糖，我们的研究结果表明，这些成分至少部分来自气管腺浆液细胞。
Glycoconjugates secreted by bovine tracheal gland serous cells in culture were characterized after incorporation of radioactive precursor [1-14C]glucosamine and stimulation with isoproterenol. Under dissociative conditions, glycoconjugates eluted in both the void and included volumes on Sepharose Cl-4B. Fractionated by anion-exchange chromatography, the high-molecular-weight (Sepharose Cl-4B; V0) glycoconjugates gave two acidic fractions eluting at 0.5 and 2.0mNaCl; low-molecular-weight glycoconjugates of the included volumes gave a neutral fraction and two acidic fractions eluting at 0.5 and 2.0mNaCl. Based on chemical analysis and specific enzymatic digestions, the material eluting in the void volume was shown to contain hyaluronic acid and chondroitin sulfate proteoglycan. In addition, the presence of small amounts of galactose, fucose, sialic acid, glucosamine, and galactosamine suggest the presence of O-glycosidically linked glycoproteins in the void volume. The identification of galactosaminitol in β-eliminated oligosaccharides from this material confirms this notion. The material eluting in the included volume was shown to contain N-linked glycoproteins with glycans of complex type in the neutral fraction and chondroitin sulfate proteoglycans in the two acidic fractions. Significant N-sulfation of amino sugars was detected in the 0.5macidic fraction, indicating the presence of heparan sulfate. Hyaluronic acid and chondroitin sulfate proteoglycan have recently been identified in tracheal secretions; our results suggest that these components originate at least in part from tracheal gland serous cells.