New P serotype of group a human rotavirus closely related to that of a porcine rotavirus
New P serotype of group a human rotavirus closely related to that of a porcine rotavirus
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DOI：
10.1002/(sici)1096-9071(200001)60:1
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发表时间：
2000-01-01
影响因子：
12.7
通讯作者：
Urasawa, S
中科院分区：
文献类型：
--
作者：
Okada, J;Urasawa, T;Urasawa, S
对1989年在泰国清迈分离到的两株具有独特血清学和基因组特性的人A组轮状病毒Mc323和Mc345的VP7和VP4基因进行了进一步鉴定[Urasawa et al. (1992) Journal of Infectious Diseases[166:227-234]。VP7基因的核苷酸和推断出的氨基酸序列使这两株菌株被归类为G9血清型。这两种病毒的VP4基因长度为2359个核苷酸，编码了一个775个氨基酸的蛋白质，就像大多数人类轮状病毒一样。将菌株Mc323的VP4氨基酸序列与菌株Mc345以及迄今报道的24种人类和动物轮状病毒的VP4序列进行比较，结果表明，Mc323和Mc345的VP4属于先前报道的猪轮状病毒的19基因型[Burke et al. (1994) Journal of General Virology 75:2205-2212]。为了研究这些VP4s的血清学型（P血清型），制备了6种重组病毒，每种重组病毒都含有人类轮状病毒的VP4基因特征和猪轮状病毒Gottfried株（G4）的VP7基因，并在家兔中制备了针对这些重组病毒的抗血清。在中和试验中，Mc323的P血清型与先前报道的人类轮状病毒的五种主要P血清型明显不同，表明Mc323和Mc345代表一种新的人类轮状病毒P血清型，暂称为P11。中华医学杂志，2006,32(3):391 - 391。(C) 2000 Wiley-Liss, Inc。
The VP7 and VP4 genes of two human group A rotavirus strains Mc323 and Mc345 with unique serologic and genomic properties, and isolated in Chiang Mai, Thailand, in 1989 [Urasawa et al. (1992) Journal of Infectious Diseases 166:227-234] were further characterized. The nucleotide and deduced amino acid sequences of the VP7 genes allowed the classification of both strains as serotype G9. The VP4 genes of both strains are 2,359 nucleotides in length and encode a protein of 775 amino acids like in most human rotaviruses. A comparison of the VP4 amino acid sequence of strain Mc323 with those of strain Mc345 and 24 human and animal rotaviruses representing 20 distinct VP4 genotypes reported to date showed that VP4 of Mc323 and Mc345 belong to genotype 19 previously reported for porcine rotavirus [Burke et al. (1994) Journal of General Virology 75:2205-2212]. To investigate the serological type (P serotype) of these VP4s, six reassortant viruses each containing a distinct VP4 gene characteristic of human rotaviruses and the VP7 gene of porcine rotavirus strain Gottfried (G4) were prepared, and antisera to these reassortants produced in rabbits. In neutralization tests, the P serotype of Mc323 was clearly differentiated from the five major P serotypes reported previously for human rotaviruses, suggesting that Mc323 and Mc345 represent a new human rotavirus P serotype tentatively called P11. J. Med. Virol. 60:63-69, 2000. (C) 2000 Wiley-Liss, Inc.