LABORATORY BLOOD FEEDING OF CULICOIDES-MISSISSIPPIENSIS (DIPTERA, CERATOPOGONIDAE) THROUGH A REINFORCED SILICONE MEMBRANE

LABORATORY BLOOD FEEDING OF CULICOIDES-MISSISSIPPIENSIS (DIPTERA, CERATOPOGONIDAE) THROUGH A REINFORCED SILICONE MEMBRANE
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DOI:
10.1093/jmedent/20.2.177
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发表时间:
1983-01-01
影响因子:
2.1
通讯作者:
KLINE, DL
KLINE, DL
中科院分区:
农林科学3区
文献类型:
--
作者:
DAVIS, EL;BUTLER, JF;KLINE, DL

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耐用硅胶喂养膜的制备和使用。描述了实验室中的密西西比色念珠菌。不含添加剂的去纤维牛血和含有0.3%柠檬酸钠的血液均成功地用于膜饲喂装置中,提供了约10%的平均饲喂百分比。 78%。在去纤维的人类宿主上喂养的雌性中,成熟卵的平均数量(40 .+-. 11、35 .+-. 12、37 .+-. 13)或卵孵化百分比(72 .+-. 5%、61 .+-. 13%、68 .+-. 6%)没有显着差异(P > 0.05)。牛血和柠檬酸牛血。
The preparation and use of a durable silicone membrane for feeding. C. mississippiensis in the laboratory are described. Defibrinated bovine blood with no additives and blood that contained 0.3% sodium citrate were each used successfully in the membrane feeding apparatus, providing a mean feeding percentage of .apprx. 78%. No significant differences (P > 0.05) were noted in mean number of eggs matured (40 .+-. 11, 35 .+-. 12, 37 .+-. 13) or in the percentage of egg hatch (72 .+-. 5%, 61 .+-. 13%, 68 .+-. 6%) among females fed, respectively, on a human host, defribrinated bovine blood and citrated bovine blood.