Validated method for the quantification of artepillin-C in Brazilian propolis
Validated method for the quantification of artepillin-C in Brazilian propolis
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DOI：
10.1002/pca.1043
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
De Almeida-Muradian, Ligia Bicudo
中科院分区：
文献类型：
--
作者：
Matsuda, Adriana Hitomi;De Almeida-Muradian, Ligia Bicudo
巴西蜂胶含有几种酚类化合物，其中5-二戊烯-4-羟基肉桂酸(青蒿素-C)常见于巴氏杆菌种类丰富的地区。青蒿素-C的定量已成为巴西蜂胶质量的重要指标，可作为出口绿色蜂胶质量控制的化学标志。这项工作是为了验证该方法，并评估从巴西不同地区收集的33份样品中青蒿素-C的含量。采用反相C-18色谱柱，紫外-可见检测。所研究的验证参数为：线性、准确度、精密度、定量和检出限。结果表明，该方法的检出限为0.0036微克/毫升，定量限为0.012微克/毫升，准确度为0.0064和0.078，回收率为98-102%。青蒿素-C的含量因产地而异，从0到11%不等。来自东南部地区的蜂胶青蒿素-C含量最高(5.0-11.0%)。来自东北地区的惠斯特没有显示任何青蒿素-C。版权所有(C)2008 John Wiley&Sons，Ltd.
Brazilian propolis contains several phenolic compounds among which 5 diprenyl-4-hydroxycinnamic acid (artepillin-C) is commonly found in areas where flora is rich in Baccharis species. The quantification of artepillin-C has become an important factor as an indicator of Brazilian propolis quality and the compound may be used as a chemical marker for quality control in exportating green propolis. This work was to validate the method and evaluate the content of artepillin-C from 33 samples collected in different Brazilian regions. The method used was HPLC with UV-vis detection and a reversed-phase C-18 Column. The validation parameters studied were: linearity, accuracy, precision, quantification and detection limits. The results obtained were: detection limit = 0.0036 mu g/mL, quantification limit = 0.012 mu g/mL, accuracy = 0.0064 and 0.078, recovery 98-102%. Artepillin-C content varied from 0 to 11% depending on the geographical origin. Propolis from the southeast region presented the highest level of artepillin-C (5.0-11.0%). Whist that from the northeast region did not show any artepillin-C. Copyright (C) 2008 John Wiley & Sons, Ltd.