Vector competence of Aedes aegypti populations from Senegal for sylvatic and epidemic dengue 2 virus isolated in West Africa
Vector competence of Aedes aegypti populations from Senegal for sylvatic and epidemic dengue 2 virus isolated in West Africa
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DOI：
10.1016/j.trstmh.2008.02.010
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发表时间：
2008-05-01
影响因子：
2.2
通讯作者：
Sall, Amadou Alpha
中科院分区：
文献类型：
--
作者：
Diallo, Mawlouth;Ba, Yamar;Sall, Amadou Alpha
对来自塞内加尔不同生物气候区和生境的6个地点的埃及伊蚊种群进行了森林（ArD 140875）和流行（ArA 6894）登革2型病毒（DENV-2）分离株的载体能力评估。用含有10（6.5）和1.6 × 10（7）组织培养感染剂量（TCID 50）的森林DENV-2分离株或10（6.5）TCID 50的流行性分离株的血肉喂养F1代蚊子，并孵育14天。在此期间之后，将每个个体的腿和翅膀合并，并单独收集相应的身体。使用免疫荧光测定法分别测试身体和翅膀/腿的DENV-2感染。结果表明：（1）塞内加尔Ae.埃及人的传病能力很强结果表明：（1）不同蚊种对登革病毒的敏感性差异不明显，感染率0- 26%，传播率10-100%，不同蚊种对登革病毒的敏感性差异不明显，影响敏感性的主要因素是病毒滴度;（iv）除Kedougou外，所有人口都能传播病毒。总的来说，我们的研究结果，白色扩展到其他四个人群，证实了以前的研究显示塞内加尔Ae的易感性低。埃及人。(C)2008年皇家热带医学和卫生学会。由爱思唯尔有限公司出版。保留所有权利。
Vector competence of Aedes aegypti populations from six locations in different bioclimatic zones and habitats of Senegal was assessed for sylvatic (ArD 140875) and epidemic (ArA 6894) dengue 2 virus (DENV-2) isolates. F1 generation mosquitoes were fed with a blood meat containing 10(6.5) and 1.6 x 10(7) tissue culture infectious dose (TCID50) of the sylvatic DENV-2 isolate or 10(6.5) TCID50 of the epidemic isolate and incubated for 14 days. After this period, Legs and wings of each individual were pooled and the corresponding body was collected separately. An immunofluorescence assay was used to test bodies and wings/legs for DENV-2 infection separately. The results showed that: (i) Senegalese Ae. aegypti populations have tower vector competence (infection rate 0-26%, dissemination rate 10-100%) for sylvatic and epidemic dengue viruses than populations from Asia and America; (ii) there was no obvious geographic variation in susceptibility among mosquito populations; (iii) the only apparent factor affecting the susceptibility was the virus titre; and (iv) except for that of Kedougou, all populations were able to disseminate the virus. Overall our findings, white extended to four other populations, confirm previous studies showing low susceptibility of Senegalese Ae. aegypti populations. (C) 2008 Royal Society of Tropical Medicine and Hygiene. Published by Elsevier Ltd. All rights reserved.