CROSS-REACTIVE AND SEROTYPE-SPECIFIC NEUTRALIZATION EPITOPES ON VP7 OF HUMAN ROTAVIRUS - NUCLEOTIDE-SEQUENCE ANALYSIS OF ANTIGENIC MUTANTS SELECTED WITH MONOCLONAL-ANTIBODIES
CROSS-REACTIVE AND SEROTYPE-SPECIFIC NEUTRALIZATION EPITOPES ON VP7 OF HUMAN ROTAVIRUS - NUCLEOTIDE-SEQUENCE ANALYSIS OF ANTIGENIC MUTANTS SELECTED WITH MONOCLONAL-ANTIBODIES
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DOI：
10.1128/jvi.62.6.1870-1874.1988
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发表时间：
1988-06-01
影响因子：
5.4
通讯作者：
GORZIGLIA, M
中科院分区：
文献类型：
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作者：
TANIGUCHI, K;HOSHINO, Y;GORZIGLIA, M
通过产生6种中和性单克隆抗体（N-MAbs）并使用这些N-MAbs来选择抵抗用于其选择的N-MAbs的中和的抗原突变体，来研究人和猴轮状病毒蛋白VP 7的中和表位。N-MAb和抗体选择的抗原突变体之间的交叉中和测试鉴定了一个交叉反应性和五个不同的血清型特异性中和表位，这些表位在操作上彼此重叠并构成单个抗原位点。此外，还鉴定了人轮状病毒VP 7中的氨基酸取代，这些氨基酸取代是用抗VP 7交叉反应性或亚型特异性N-MAb选择的突变体中抗原性改变的原因。抗原突变体中的所有氨基酸取代都发生在两个可变区域之一：氨基酸87至101和208至221。
The neutralization epitopes of human and simian rotavirus protein VP7 were studied by producing six neutralizing monoclonal antibodies (N-MAbs) and using these N-MAbs to select antigenic mutants that resisted neutralization by the N-MAbs used for their selection. Cross-neutralization tests between the N-MAbs and the antibody-selected antigenic mutants identified one cross-reactive and five distinct serotype-specific neutralization epitopes which operationally overlapped one another and constituted a single antigenic site. In addition the amino acid substitutions in human rotavirus VP7 that are responsible for the antigenic alterations in the mutants selected with anti-VP7 cross-reactive or serotype-specific N-MAbs were identified. All the amino acid substitutions in the antigenic mutants occurred in one of two varible regions: amino acids 87 to 101 and 208 to 221.