Persistence of Borrelia burgdorferi sensu lato in resolved erythema migrans lesions.
Persistence of Borrelia burgdorferi sensu lato in resolved erythema migrans lesions.
复制标题
伯氏疏螺旋体在已解决的游走性红斑病变中的持续存在。
DOI：
10.1093/clinids/21.2.380
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Picken,RN
中科院分区：
文献类型：
--
作者：
Strle,F;Cheng,Y;Cimperman,J;Maraspin,V;Lotric-Furlan,S;Nelson,JA;Picken,MM;Ruzic-Sabljic,E;Picken,RN
由蜱叮咬和伯氏疏螺旋体感染引起的游走性红斑皮肤病变最终会消退，即使没有抗生素治疗。本研究的目的是测量B. burgdorferisensu lato在这种病变。因此，对48名患者的既往病变部位进行了活检和培养：39名患者有全身或局限性淋巴结转移征，9名患者无淋巴结转移征。从三名有症状的患者的活组织检查皮肤中分离出广泛的Burgdorferisensu lato。其他组织和液体培养均为阴性。经基因种特异性聚合酶链反应（PCR）和脉冲场凝胶电泳（PFGE）鉴定，2株分离株均为阿氏疏螺旋体（Borrelia afzelii）。剩下的分离株是一个不寻常的asB型菌株组的成员。burgdorferisensu stricto，但具有非典型PFGE图谱。所有三名患者对抗生素治疗都有显著的临床反应。这些发现证明了viableB的能力。Burgdorferisensu lato微生物在消退的游走性红斑皮疹部位的临床表现正常的皮肤中持续2个月至3.5年。这一观察结果可能为生物体逃避宿主免疫反应的能力提供新的见解。
Erythema migrans skin lesions resulting from a tick bite and infection withBorrelia burgdorferisensu lato eventually resolve, even without antibiotic therapy. The aim of the present study was to gauge the frequency of persistence ofB. burgdorferisensu lato in such lesions. Thus, the site of a previous lesion was biopsied and cultured in 48 patients: 39 with systemic or localized symptomsl signs and nine with no symptoms.B. burgdorferisensu lato was isolated from biopsied skin from three symptomatic patients. Cultures of other tissues and fluids were negative. By genospecies-specific polymerase chain reaction (PCR) and pulsed-field gel electrophoretic (PFGE) analysis, two isolates were classified asBorrelia afzelii. The remaining isolate was a member of an unusual group of strains that type asB. burgdorferisensu stricto by genospecies PCR but possess an atypical PFGE profile. All three patients had a dramatic clinical response to antibiotic treatment. These findings demonstrate the capacity of viableB. burgdorferisensu lato organisms to persist in clinically normal-appearing skin at the site of a resolved erythema migrans rash for periods ranging from 2 months to 3.5 years. This observation may provide new insight into the organisms' ability to evade the host's immune response.