Neural responses to territorial challenge and nonsocial stress in male song sparrows:: segregation, integration, and modulation by a vasopressin V1 antagonist
Neural responses to territorial challenge and nonsocial stress in male song sparrows:: segregation, integration, and modulation by a vasopressin V1 antagonist
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DOI：
10.1016/j.yhbeh.2004.02.008
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发表时间：
2004-11-01
影响因子：
3.5
通讯作者：
Evans, AK
中科院分区：
文献类型：
--
作者：
Goodson, JL;Evans, AK
本实验旨在确定(1)哪些基底前脑区域和/或其肽能成分对社会挑战和非社会压力有反应，以及(2)精氨酸抗利尿激素V-1拮抗剂（AVPa）对这些反应的影响。实验以野外捕获的雄性鸣麻雀（Melospiza melodia）为对象，将其安置在半自然区域（野外飞行笼）。每个受试者都安装了一个指向侧脑室的慢性引导插管，并在牺牲和组织化学之前暴露于五种条件中的一种：盐水+模拟领土入侵（STI，由歌曲播放和笼中同一雄性组成），AVPa + STI，盐水+空笼子，AVPa +空笼子，未处理。制备两个组织系列，免疫荧光双标记ZENK （egr-1）蛋白和精氨酸血管催产素（AVT； AVP的禽同源物）或促肾上腺皮质激素释放因子（CRF）。结果表明，对非社会压力（捕获、处理和输注）和STI敏感的神经元群体至少是部分分离的。在处理和输注后，在许多区域观察到zenk免疫反应（-ir）核的增加，而STI特异性的神经反应则更为有限。然而，多个地区对处理和STI都有反应。AVPa输注显著降低或消除了大多数实验中ZENK-ir的增加，这表明内源性AVT在调节基线活动和/或应激反应中发挥了广泛的作用，而在对社会挑战的特定反应中发挥的作用则要有限得多。特别注意外侧隔（LS）的许多区域，这些区域对处理，STI和v -1样受体阻断的反应不同。这些数据表明，间隔AVT调节对一般压力源的神经反应，而不是特定的社会刺激。因此，间隔AVT功能的物种差异（如先前在鸣禽中描述的）可能反映了压力或焦虑与物种特定行为或物种典型环境下行为的关系差异。(C) 2004爱思唯尔公司版权所有。
The present experiments were conducted to determine (1) which basal forebrain regions and/or their peptidergic components are responsive to social challenge and nonsocial stress, and (2) the influence of an arginine vasopressin V-1 antagonist (AVPa) on these responses. Experiments were conducted in wild-caught male song sparrows (Melospiza melodia) that were housed on seminatural territories (field-based flight cages). Subjects were each fitted with a chronic guide cannula directed at the lateral ventricle and exposed to one of five conditions before sacrifice and histochemistry: saline + simulated territorial intrusion (STI; consisting of song playback and presentation of a caged conspecific male), AVPa + STI, saline + empty cage, AVPa + empty cage, unhandled. Two tissue series were prepared and immunofluorescently double-labeled for ZENK (egr-1) protein and either arginine vasotocin (AVT; avian homologue of AVP) or corticotropin releasing factor (CRF). The results indicate that the neuronal populations that are sensitive to nonsocial stress (capture, handling and infusion) and STI are at least partially segregated. Increases in ZENK-immunoreactive (-ir) nuclei following handling and infusion were observed in a large number of areas, whereas neural responses that were specific to STI were more limited. However, multiple areas showed responses to both handling and STI. AVPa infusions significantly reduced or eliminated most experimental increases in ZENK-ir, suggesting a broad role for endogenous AVT in the modulation of baseline activity and/or stress responsivity, and a much more limited role in the specific response to social challenge. Particular attention is given to the numerous zones of the lateral septum (LS), which are differentially responsive to handling, STI, and V-1-like receptor blockade. These data suggest that septal AVT modulates neural responses to general stressors, not social stimuli specifically. Thus, species differences in septal AVT function (as previously described in songbirds) likely reflect differences in the relationship of stress or anxiety to species-specific behaviors, or to behavior in species-typical contexts. (C) 2004 Elsevier Inc. All rights reserved.