Simple and rapid procedure for the selective removal of lysozyme from human saliva

Simple and rapid procedure for the selective removal of lysozyme from human saliva
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简单快速地从人唾液中选择性去除溶菌酶

DOI:
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发表时间:
1979
影响因子:
3.1
通讯作者:
L. Tellefson
L. Tellefson
中科院分区:
医学2区
文献类型:
--
作者:
G. Germaine;L. Tellefson

文献摘要

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本文介绍了一种从人全唾液上清液中去除溶菌酶的简便、快速的方法。唾液标本用溶壁微球菌吸附。如此处理的唾液消耗了95%的溶菌酶活性。总蛋白、乳过氧化物酶、乳铁蛋白、免疫球蛋白A和几种阴离子蛋白比例的变化均小于10%。结论:唾液对M. lysodeikticus是制备选择性缺乏溶菌酶的唾液的合适方法。
A simple and rapid method is described for the removal of lysozyme from human whole salivary supernatant. Saliva specimens were adsorbed with Micrococcus lysodeikticus. The saliva so treated was depleted of 95% of the lysozyme activity. Changes in total protein, lactoperoxidase, lactoferrin, immunoglobulin A, and the proportions of several anionic proteins were less than 10%. It is concluded that adsorption of saliva with M. lysodeikticus is a suitable procedure for the preparation of saliva that is selectively deficient in lysozyme.