Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies.
Regulation of immune responses by I-J gene products. I. Production and characterization of anti-I-J monoclonal antibodies.
复制标题
DOI：
10.1084/jem.154.5.1570
复制
发表时间：
1981-11-01
期刊：
影响因子：
--
通讯作者：
Waltenbaugh C
中科院分区：
文献类型：
--
作者：
Waltenbaugh C
使用一种新的两步功能筛选程序，我们分离出杂交瘤B细胞系，分泌针对小鼠H-2复合体I-Jb和I-Jk亚区基因产物的单克隆抗体。这些单克隆抗体在体外通过使无应答的脾细胞对正常抑制量的poly(Glu50Tyr50) (GT) （WF8系列抗i- jk单克隆抗体）或次优浓度的poly(Glu60Ala30Tyr10) （WF9系列抗i- jb单克隆抗体）产生应答而起作用。一些培养上清显示出与gt特异性T细胞衍生抑制因子（GT-TsF）结合的增强活性，表明一些单克隆抗抗体显示出非特异性增强作用，或者，更有可能的是，抗I-J单克隆抗体已经产生，以对抗在TsF上未发现的I-J决定因子。最后一种可能性是最有趣的，它可以作为探索I-J分区域异质性的一种手段。我们的一些单克隆抗i- j抗体也可能检测选择性表达在抑制性T细胞、辅助性T细胞和/或巨噬细胞上的抗原决定因子。此外，我们已经证明单克隆抗i- j抗体在单克隆GT-TsF的生化表征和纯化中应该是有用的。这些单倍型特异性抗I-J单克隆抗体将被证明是未来研究I-J基因产物在特异性免疫反应调控中的作用的有力工具。
Using a novel, two-step functional screening procedure, we have isolated hybridoma B cell lines secreting monoclonal antibodies directed against gene products of the I-Jb and I-Jk subregions of the mouse H-2 complex. These monoclonal antibodies act in vitro by allowing nonresponder spleen cells to respond to normally suppressive quantities of poly(Glu50Tyr50) (GT) (WF8 series of anti-I-Jk monoclonal antibodies) or to suboptimal concentration of poly(Glu60Ala30Tyr10) (WF9 series of anti-I-Jb monoclonal antibodies). Some of the culture supernates that show augmenting activity bind GT-specific T cell- derived suppressor factor (GT-TsF), indicating that some monoclonal antiantibodies display a nonspecific enhancing effect, or, more likely, that anti-I-J monoclonal antibodies have been produced against I-J determinants not found on TsF. It is this last possibility that is most intriguing and that might serve as a means for exploring the heterogeneity of the I-J subregion. It is also possible that some of our monoclonal anti-I-J antibodies might detect antigenic determinants selectively expressed on suppressor T cells, helper T cells, and/or macrophages. In addition, we have demonstrated that monoclonal anti-I-J antibodies should be useful in the biochemical characterization and purification of a monoclonal GT-TsF. These haplotype-specific anti-I-J monoclonal antibodies should prove to be powerful tools for future studies exploring the role of I-J gene products in the regulation of specific immune responses.