Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?
Ribonucleic artefacts: are some extracellular RNA discoveries driven by cell culture medium components?
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DOI：
10.1080/20013078.2016.1272832
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发表时间：
2017
影响因子：
16
通讯作者：
Witwer KW
中科院分区：
文献类型：
--
作者：
Tosar JP;Cayota A;Eitan E;Halushka MK;Witwer KW
在最近发表的一项研究中，安娜克里切夫斯基及其同事提出了一个重要的问题，即体外细胞外RNA（exRNA）研究（包括细胞外囊泡（EV）研究）的结果是否会受到细胞培养基成分（如胎牛血清（FBS））中RNA存在的干扰。根据他们的数据，答案是一个响亮的“是”。即使在从FBS中去除牛EV的长时间超离心后，FBS中的大部分exRNA仍然存在。尽管技术因素可能影响消耗的程度，但FBS中残留的EV和exRNA可能影响体外研究的结论：当然，对于分泌的RNA，也可能对于细胞相关RNA。在这篇评论中，我们批判性地研究了这一领域的一些文献，包括这篇文章的一些作者最近的一项研究，根据Wei等人的研究，并探索细胞培养衍生的RNA如何影响我们对EV RNA的了解。随着该领域对EV-RNA关联和潜在功能的更深入理解，这些发现具有特殊的意义。
In a recently published study, Anna Krichevsky and colleagues raise the important question of whether results of in vitro extracellular RNA (exRNA) studies, including extracellular vesicle (EV) investigations, are confounded by the presence of RNA in cell culture medium components such as foetal bovine serum (FBS). The answer, according to their data, is a resounding “yes”. Even after lengthy ultracentrifugation to remove bovine EVs from FBS, the majority of exRNA in FBS remained. Although technical factors may affect the degree of depletion, residual EVs and exRNA in FBS could influence the conclusions of in vitro studies: certainly, for secreted RNA, and possibly also for cell-associated RNA. In this commentary, we critically examine some of the literature in this field, including a recent study from some of the authors of this piece, in light of the Wei et al. study and explore how cell culture-derived RNAs may affect what we think we know about EV RNAs. These findings hold particular consequence as the field moves towards a deeper understanding of EV–RNA associations and potential functions.