Variation in epitopes of the B subunit of El Tor and classical biotype Vibrio cholerae O1 cholera toxin.
Variation in epitopes of the B subunit of El Tor and classical biotype Vibrio cholerae O1 cholera toxin.
复制标题
El Tor B 亚基和经典生物型霍乱弧菌 O1 霍乱毒素的表位变异。
DOI：
10.1099/00221287-135-5-1195
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Colwell,RR
中科院分区：
文献类型：
--
作者：
Tamplin,ML;Ahmed,MK;Jalali,R;Colwell,RR
用制备的单克隆抗体检测El - Tor菌株和经典生物型霍乱弧菌o1菌株产生的霍乱毒素（CT-B） B亚基表位的变异。cholerae569B CT。CT-B表位对v明显保守。choleraeclassical生物型。相比之下，El Tor生物型的表位变异被观察到，它产生一个经典的CT-B和一个独特的CT-B，缺乏至少一个569B CT-B共有的表位。缺失的表位位于gm1神经节苷脂结合位点之外。从这里报道的研究结果来看，El Tor生物型CT-B与经典CT-B表现出遗传差异。此外，至少有5个独特的v。cholerae569B CT-B可定义。
Variation in epitopes of the B subunit of cholera toxin (CT-B) produced by strains of El Tor and classical biotypeVibrio choleraeO1 was examined using monoclonal antibodies prepared toV. cholerae569B CT. CT-B epitopes were markedly conserved forV. choleraeclassical biotypes. In contrast, epitope variation was observed for El Tor biotypes, which produced both a classical-like CT-B and a unique CT-B lacking at least one epitope common to 569B CT-B. The missing epitope was located outside the GM1ganglioside-binding site. From results of the study reported here, genetic divergence is exhibited in the El Tor biotype CT-B versus classical CT-B. Furthermore, at least five unique epitopes ofV. cholerae569B CT-B can be defined.