Tetraspanin protein (TSP-15) integrity is required for epidermal integrity in Caenorhabditis elegans
Tetraspanin protein (TSP-15) integrity is required for epidermal integrity in Caenorhabditis elegans
复制标题
DOI：
10.1242/jcs.01403
复制
发表时间：
2004-10-15
影响因子：
4
通讯作者：
Mekada, E
中科院分区：
文献类型：
--
作者：
Moribe, H;Yochem, J;Mekada, E
表皮完整性对于动物的发育和生存至关重要。在这里，我们证明 TSP-15（四跨膜蛋白家族的成员）是秀丽隐杆线虫上皮膜完整性所必需的。通过突变或RNA干扰降低tsp-15功能会引起皮下组织异常，包括角质层解离和皮下组织退化。蜕皮过程中常常会导致死亡。对 GFP 转基因动物的检查、遗传嵌合分析和救援分析表明，TSP-15 在 hyp7（构成大部分皮下组织的大型合胞体）中发挥作用。膜不可渗透染料的测定或皮下特异性标记物的渗漏分析表明，皮下膜的屏障功能由于 TSP-15 的损失或减少而受损。这些结果表明 TSP-15 在维持上皮细胞完整性方面发挥作用。
Epidermal integrity is essential for animal development and survival. Here, we demonstrate that TSP-15, a member of the tetraspanin protein family, is required for epithelial membrane integrity in Caenorhabditis elegans. Reduction of tsp-15 function by mutation or by RNA interference elicits abnormalities of the hypodermis, including dissociation of the cuticle and degeneration of the hypodermis. Lethality during molting often results. Examination of GFP transgenic animals, genetic mosaic analysis and rescue assays revealed that TSP-15 functions in hyp7, a large syncytium that composes most of the hypodermis. Assays with a membrane-impermeable dye or leakage analysis of a hypodermal-specific marker indicate that the barrier function of the hypodermal membrane is impaired owing to the loss or reduction of TSP-15. These results indicate that TSP-15 functions in the maintenance of epithelial cell integrity.