Identification of hundreds of conserved and nonconserved human microRNAs
Identification of hundreds of conserved and nonconserved human microRNAs
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DOI：
10.1038/ng1590
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发表时间：
2005-07-01
期刊：
影响因子：
30.8
通讯作者：
Bentwich, Z
中科院分区：
文献类型：
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作者：
Bentwich, I;Avniel, A;Bentwich, Z
MicroRNA是与22个核苷酸相似的非编码RNA，通过与其mRNA结合，抑制靶基因的翻译，因此在健康和疾病中的基因调节中具有核心作用（1-5）。迄今为止，已经鉴定出222个人MicroRNA（6），通过随机克隆和测序鉴定了86个，43通过计算方法，其余的作为其他物种中与microRNA同源的假定microRNA。为了证明我们的假设，即microRNA的总数可能更大，并且只有一些在灵长类动物中才出现，我们开发了一种联合方法，将生物信息学预测与微阵列分析和序列定向的克隆相结合。在这里，我们报告了这种方法在克隆和序列89新的人类microRNA（几乎增加了测序的人MicroRNA的数量），其中53个并不是灵长类动物以外的保守。这些发现表明，人类microRNA的总数至少为800。
MicroRNAs are noncoding RNAs of similar to 22 nucleotides that suppress translation of target genes by binding to their mRNA and thus have a central role in gene regulation in health and disease(1-5). To date, 222 human microRNAs have been identified(6), 86 by random cloning and sequencing, 43 by computational approaches and the rest as putative microRNAs homologous to microRNAs in other species. To prove our hypothesis that the total number of microRNAs may be much larger and that several have emerged only in primates, we developed an integrative approach combining bioinformatic predictions with microarray analysis and sequence- directed cloning. Here we report the use of this approach to clone and sequence 89 new human microRNAs ( nearly doubling the current number of sequenced human microRNAs), 53 of which are not conserved beyond primates. These findings suggest that the total number of human microRNAs is at least 800.