Rapid consolidation to a radish and protein synthesis-dependent long-term memory after single-session appetitive olfactory conditioning in Drosophila
Rapid consolidation to a radish and protein synthesis-dependent long-term memory after single-session appetitive olfactory conditioning in Drosophila
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DOI：
10.1523/jneurosci.5333-07.2008
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发表时间：
2008-03-19
影响因子：
5.3
通讯作者：
Waddell, Scott
中科院分区：
文献类型：
--
作者：
Krashes, Michael J.;Waddell, Scott
在果蝇中，厌恶性嗅觉长期记忆（LTM）的形成需要多次训练，将气味和电击惩罚与休息间隔结合起来。相比之下，我们在这里表明，将气味与行为学上更相关的糖强化配对的单一 2 分钟训练课程可以形成持续数天的长期食欲记忆。食欲性 LTM 与厌恶性 LTM 在机制上有一些相似之处，因为它可以被放线菌酮、dCreb2-b 转录抑制因子以及 crammer 和龙舌兰酒 LTM 特异性突变所破坏。然而，食欲的 LTM 被萝卜突变完全破坏了，萝卜突变显然代表了巩固厌恶记忆的一个独特的机制阶段。此外，食欲 LTM 需要训练后第一个小时内背侧成对的内侧神经元和蘑菇体 α'β' 神经元回路的活动以及检索期间蘑菇体 αβ 神经元的输出，这表明食欲中期记忆和 LTM 在机制上是相关的。最后，训练后喂养和/或饥饿果蝇的实验揭示了实现 LTM 食欲检索的关键动机驱动力。
In Drosophila, formation of aversive olfactory long-term memory (LTM) requires multiple training sessions pairing odor and electric shock punishment with rest intervals. In contrast, here we show that a single 2 min training session pairing odor with a more ethologically relevant sugar reinforcement forms long-term appetitive memory that lasts for days. Appetitive LTM has some mechanistic similarity to aversive LTM in that it can be disrupted by cycloheximide, the dCreb2-b transcriptional repressor, and the crammer and tequila LTM-specific mutations. However, appetitive LTM is completely disrupted by the radish mutation that apparently represents a distinct mechanistic phase of consolidated aversive memory. Furthermore, appetitive LTM requires activity in the dorsal paired medial neuron and mushroom body alpha'beta' neuron circuit during the first hour after training and mushroom body alpha beta neuron output during retrieval, suggesting that appetitive middle-termmemory and LTM are mechanistically linked. Last, experiments feeding and/or starving flies after training reveals a critical motivational drive that enables appetitive LTM retrieval.