Fibronectin (LETS) does not support epithelial cell spreading.
Fibronectin (LETS) does not support epithelial cell spreading.
复制标题
纤连蛋白 (LETS) 不支持上皮细胞扩散。
DOI：
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发表时间：
1980
影响因子：
6.5
通讯作者：
K. Stenn
中科院分区：
文献类型：
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作者：
W. Federgreen;K. Stenn
使用纯化的人血浆纤连蛋白和两种体外试验研究了上皮细胞铺展对含纤连蛋白培养基的依赖性：（1）来自小鼠耳皮肤测量片的表皮生长，和（2）来自胰蛋白酶化豚鼠表皮的解离细胞铺展。使用这些系统，在含有全血浆和缺乏纤连蛋白的血浆的培养基中发生了明显的扩散，但在仅富含纤连蛋白的培养基中没有发生显著的扩散。将纤连蛋白返回到缺乏的血浆中不会增强或减少最佳细胞铺展。这些研究表明，人血浆中支持上皮细胞体外扩散的因子不是纤连蛋白，上皮细胞和成纤维细胞扩散的化学性质不同。
The dependence of epithelial cell spreading on fibronectin-containing media has been investigated, using purified human plasma fibronectin, and two in vitro assays: (1) epidermal outgrowths from measured pieces of mouse ear skin, and (2) spreading of dissociated cells from trypsinized guinea pig epidermis. Using these systems, appreciable spread occurred in media containing whole plasma and plasma deficient in fibronectin but no significant spread occurred in media enriched only in fibronectin. Returning fibronectin to deficient plasma did not enhance or reduce optimal cell spreading. These studies suggest that the factor(s) in human plasma which supports epithelial cell spreading in vitro is not fibronectin and that the chemistry of epithelial and fibroblast cell spreading is different.