High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider communities
High-throughput sequencing for community analysis: the promise of DNA barcoding to uncover diversity, relatedness, abundances and interactions in spider communities
复制标题
DOI：
10.1007/s00427-020-00652-x
复制
发表时间：
2020-02-10
影响因子：
2.4
通讯作者：
Krehenwinkel, Henrik
中科院分区：
文献类型：
--
作者：
Kennedy, Susan R.;Prost, Stefan;Krehenwinkel, Henrik
对群落生态学的大规模研究是非常需要的，但往往难以完成，因为需要大量的时间、劳动力和金钱来表征丰富度、丰富度、相关性和相互作用。尽管如此，这种大规模的视角对于理解生物群落的组成、动态和弹性是必要的。小型无脊椎动物在生态系统中发挥着核心作用，在食物网中占据着关键位置，并发挥着各种各样的生态功能。然而，由于这些动物的高度多样性和丰富性，对它们的群落进行充分的描述尤其困难。尤其是蜘蛛，在陆生食物网中扮演着捕食者和猎物的双重角色，因此成为生态学研究的重要焦点。近年来，DNA条形码技术的进步使大规模的群落分析受益匪浅。高通量测序（HTS），特别是DNA元条形码，能够以前所未有的规模和以前可能的一小部分成本，在社区范围内分析多样性和相互作用。在这里，我们回顾了这些技术在蜘蛛群落分析中的应用现状。我们讨论了基于扩增子的DNA条形码和元条形码用于群落多样性分析，以及用于评估捕食者-猎物关系的肠道分子含量分析。我们还重点介绍了第三代测序技术在长读和便携式DNA条形码中的应用。然后，我们讨论了社区水平研究的理论框架的发展，最后强调了蜘蛛群落DNA分析的关键差距和未来方向。
Large-scale studies on community ecology are highly desirable but often difficult to accomplish due to the considerable investment of time, labor and, money required to characterize richness, abundance, relatedness, and interactions. Nonetheless, such large-scale perspectives are necessary for understanding the composition, dynamics, and resilience of biological communities. Small invertebrates play a central role in ecosystems, occupying critical positions in the food web and performing a broad variety of ecological functions. However, it has been particularly difficult to adequately characterize communities of these animals because of their exceptionally high diversity and abundance. Spiders in particular fulfill key roles as both predator and prey in terrestrial food webs and are hence an important focus of ecological studies. In recent years, large-scale community analyses have benefitted tremendously from advances in DNA barcoding technology. High-throughput sequencing (HTS), particularly DNA metabarcoding, enables community-wide analyses of diversity and interactions at unprecedented scales and at a fraction of the cost that was previously possible. Here, we review the current state of the application of these technologies to the analysis of spider communities. We discuss amplicon-based DNA barcoding and metabarcoding for the analysis of community diversity and molecular gut content analysis for assessing predator-prey relationships. We also highlight applications of the third generation sequencing technology for long read and portable DNA barcoding. We then address the development of theoretical frameworks for community-level studies, and finally highlight critical gaps and future directions for DNA analysis of spider communities.