Effects of rhizobacteria on parasitism by Meloidogyne ethiopica on grapevines
Effects of rhizobacteria on parasitism by Meloidogyne ethiopica on grapevines
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DOI：
10.1007/s10658-012-0073-7
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发表时间：
2013-01-01
影响因子：
1.8
通讯作者：
Persson, Paula
中科院分区：
文献类型：
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作者：
Aballay, Erwin;Ordenes, Paula;Persson, Paula
大豆根结线虫是智利最重要的植物寄生线虫之一，侵袭性强，防治难度大。本研究评价了从葡萄根部分离的16株根际细菌对盆栽藤本植物的寄生和线虫危害的影响。通过对2个月生的仙人掌植株的处理，评价了根际细菌的拮抗效果。用含有1×10(6)cfu ml(-1)细菌和1,000个线虫卵的悬浮液在3-L盆中放置霞多丽。在生长6个月后，切下植株，测定根和冠层重量、线虫种群和根部损害。此外，还研究了根际细菌培养滤液对线虫卵孵化的影响。7株根际细菌被证明能有效地抑制线虫的破坏或繁殖。这些菌株分别是粘质沙雷氏菌、嗜酸粒单胞菌、聚集性泛菌、螺旋鞘氨酸杆菌、芽孢杆菌、产碱杆菌和多聚沙雷氏菌。另外三个菌株，巨型芽孢杆菌、聚集性芽孢杆菌和野生型假单胞菌显著增加了根重，但没有减少线虫危害和种群密度。浸泡24 h后，所有菌株的上清液均显著抑制幼虫的孵化，其中恶臭假单胞菌和极大拟青霉的效果最好。
Meloidogyne ethiopica is one of the most important plant-parasitic nematodes affecting vines in Chile and is very aggressive and difficult to control. This study evaluated 16 strains of rhizobacteria, originally isolated from roots of grapevines, for their effects on parasitism and nematode damage to potted vine plants. The antagonistic effect of rhizobacteria was assessed by treating 2-month-old plants of cv. Chardonnay in 3-l pots with a suspension containing 1 x 10(6) cfu ml(-1) of the bacteria and 1,000 nematode eggs. After 6 months of growth, the plants were cut and root and canopy weights, nematode populations and root damage determined. The effect of rhizobacterial culture filtrate on hatching of nematode eggs was also assessed in vitro. Seven strains of rhizobacteria proved effective in inhibiting damage or reproduction of the nematode. These were strains of Serratia marcescens, Comamonas acidovorans, Pantoea agglomerans, Sphingobacterium spiritivorum, Bacillus mycoides, Alcaligenes piechaudii and Serratia plymuthica. A further three strains, of Bacillus megaterium, P. agglomerans and Pseudomonas savastanoi, significantly increased root weight, but did not decrease nematode damage or population density. The supernatant of all strains significantly decreased hatching of juvenile nematodes after 24 h of immersion, with isolates of P. putida and B. megaterium being the most effective.