PIQA: pipeline for Illumina G1 genome analyzer data quality assessment
PIQA: pipeline for Illumina G1 genome analyzer data quality assessment
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DOI：
10.1093/bioinformatics/btp429
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发表时间：
2009-09-15
期刊：
影响因子：
5.8
通讯作者：
Fofanov, Y.
中科院分区：
文献类型：
--
作者：
Martinez-Alcantara, A.;Ballesteros, E.;Fofanov, Y.
PIQA是一种质量分析管道，旨在检查由下一代测序技术（Illumina G1基因组分析仪）产生的基因组读数。一个简短的统计总结，以及集群密度，质量分数和核苷酸频率的逐块和逐循环的图形表示允许容易地识别各种技术问题，包括有缺陷的瓦片，样品/文库制备中的错误和测序的基因组读数出现频率的异常。PIQA是用R统计编程语言编写的，与bustard、fastq和scarf Illumina G1基因组分析仪数据格式兼容。
PIQA is a quality analysis pipeline designed to examine genomic reads produced by Next Generation Sequencing technology (Illumina G1 Genome Analyzer). A short statistical summary, as well as tile-by-tile and cycle-by-cycle graphical representation of clusters density, quality scores and nucleotide frequencies allow easy identification of various technical problems including defective tiles, mistakes in sample/library preparations and abnormalities in the frequencies of appearance of sequenced genomic reads. PIQA is written in the R statistical programming language and is compatible with bustard, fastq and scarf Illumina G1 Genome Analyzer data formats.