Autophagy contributes to regulation of the hypoxia response during submergence in Arabidopsis thaliana.
Autophagy contributes to regulation of the hypoxia response during submergence in Arabidopsis thaliana.
复制标题
自噬有助于调节拟南芥淹没过程中的缺氧反应
DOI：
10.1080/15548627.2015.1112483
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发表时间：
2015
期刊：
影响因子：
13.3
通讯作者：
Xiao S
中科院分区：
文献类型：
--
作者：
Chen L;Liao B;Qi H;Xie LJ;Huang L;Tan WJ;Zhai N;Yuan LB;Zhou Y;Yu LJ;Chen QF;Shu W;Xiao S
自噬涉及细胞内成分的大量降解，并作为保守系统发挥作用，帮助细胞适应不利条件。在哺乳动物中，缺氧迅速刺激自噬作为细胞存活反应。在这里，我们研究了自噬在植物对淹没的反应的调节中的功能，淹没是一种非生物胁迫，导致植物细胞缺氧和无氧呼吸。在拟南芥中，淹没诱导自噬相关基因（ATG）的转录和自噬体的形成。与此相一致的是，自噬缺陷（atg）突变体对淹没应激和无氧呼吸的最终产物乙醇处理非常敏感。浸水后，atg突变体的无氧呼吸基因（乙醇脱氢酶1，ADH 1和丙酮酸脱羧酶1，PDC 1）的转录水平增加，但其他缺氧和乙烯反应基因的转录水平降低。浸水和乙醇处理诱导的活性氧（ROS）的积累，在轮虫的atg突变体比野生型。此外，烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶的ROS的生产是必要的植物耐淹和乙醇，淹诱导的表达ADH 1和PDC 1，和自噬的激活。在atg突变体的淹没和乙醇敏感的表型依赖于一个完整的水杨酸（SA）信号通路。总之，我们的研究结果表明，淹没诱导的自噬功能在拟南芥的缺氧反应，通过调节SA介导的细胞内稳态。
Autophagy involves massive degradation of intracellular components and functions as a conserved system that helps cells to adapt to adverse conditions. In mammals, hypoxia rapidly stimulates autophagy as a cell survival response. Here, we examine the function of autophagy in the regulation of the plant response to submergence, an abiotic stress that leads to hypoxia and anaerobic respiration in plant cells. In Arabidopsis thaliana, submergence induces the transcription of autophagy-related (ATG) genes and the formation of autophagosomes. Consistent with this, the autophagy-defective (atg) mutants are hypersensitive to submergence stress and treatment with ethanol, the end product of anaerobic respiration. Upon submergence, the atg mutants have increased levels of transcripts of anaerobic respiration genes (alcohol dehydrogenase 1, ADH1 and pyruvate decarboxylase 1, PDC1), but reduced levels of transcripts of other hypoxia- and ethylene-responsive genes. Both submergence and ethanol treatments induce the accumulation of reactive oxygen species (ROS) in the rosettes of atg mutants more than in the wild type. Moreover, the production of ROS by the nicotinamide adenine dinucleotide phosphate (NADPH) oxidases is necessary for plant tolerance to submergence and ethanol, submergence-induced expression of ADH1 and PDC1, and activation of autophagy. The submergence- and ethanol-sensitive phenotypes in the atg mutants depend on a complete salicylic acid (SA) signaling pathway. Together, our findings demonstrate that submergence-induced autophagy functions in the hypoxia response in Arabidopsis by modulating SA-mediated cellular homeostasis.