Multi-copy genetic screen in Aspergillus nidulans.
Multi-copy genetic screen in Aspergillus nidulans.
复制标题
DOI：
10.1007/978-1-62703-122-6_13
复制
发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Yu, Jae-Hyuk
中科院分区：
文献类型：
--
作者：
Park, Hee Soo;Yu, Jae-Hyuk
随着各种曲霉属物种基因组的完成，可以进行大规模的全基因组表达研究。然而，基因组学与遗传学相结合时更强大，更有效。基于多拷贝的功能获得性筛选是功能丧失性遗传筛选的补充方法，并且可以鉴定通过功能丧失型筛选可能不容易鉴定的新基因。特别是，功能获得性基因筛选将鉴定真菌发育和次级代谢的新激活剂或抑制剂。在这里，我们描述了一个工作协议，用于识别构巢曲霉中的新型调节剂。
With the completion of genomes of various Aspergillus species, large-scale genome-wide expression studies can be carried out. Genomics, however, is more powerful and efficient when combined with genetics. A multi-copy-based gain-of-function screen is a complementary method to loss-of-function genetic screen and can identify novel genes that may not be easily identifiable through loss-of-function-type screens. Particularly, gain-of-function genetic screens would identify novel activators or repressors of fungal development and secondary metabolism. Here, we describe a working protocol for the identification of novel regulators in Aspergillus nidulans.