Pseudomonas seleniipraecipitatus sp. nov.: A Selenite Reducing γ-Proteobacteria Isolated from Soil
Pseudomonas seleniipraecipitatus sp. nov.: A Selenite Reducing γ-Proteobacteria Isolated from Soil
复制标题
Pseudomonas seleniipraecipitatus sp. nov.：从土壤中分离的亚硒酸盐还原 γ-变形菌
作者：
W. Hunter;D. Manter
从土壤中分离到一株能将亚硒酸盐还原为元素红硒的革兰氏阴性黄色细菌，命名为菌株CA 5 T。16 S rRNA基因序列比对鉴定该分离物为一种新的假单胞菌属物种，与阿根廷假单胞菌、黄假单胞菌和禾本科假单胞菌是其最近的亲属。序列比对结果表明，菌株CA 5 T的16 S rRNA基因与阿根廷假单胞菌、黄花假单胞菌和禾本科假单胞菌的16 S rRNA基因的差异分别为1.3%、1.5%和1.7%。G+C含量为62.8 mol%，与报道的黄毛原花青素的62.7-63 mol%相似，但略高于麻花原花青素的62.5-62.6 mol%，并显著高于阿根廷原花青素的57.5-58.0 mol%。CA 5 T菌株中存在的主要细胞脂肪酸为C18：1ω 7 c（41.1%）、C16：1ω6c和C16：1ω 7 c（25.7%）、C16：0（12.0%）、C12：0（8.0%）、C12：0 3-OH（4.4%）和C10：0 3-OH（2.9%）。细胞脂肪酸谱，GC含量，表型特性和生化特性与其在假单胞菌属内的位置一致。命名为P. seleniipraecipitatus。
A Gram-negative, yellow pigmented bacterium designated strain CA5T that reduced selenite to elemental red selenium was isolated from soil. 16S rRNA gene sequence alignment identified the isolate as a novel Pseudomonas species with P. argentinensis, P. flavescens, and P. straminea as its closest relatives. Sequence alignments show that the 16S rRNA gene of strain CA5T differed from that of P. argentinensis, P. flavescens, and P. straminea by 1.3, 1.5, and 1.7%, respectively. The G+C content was 62.8 mol%, similar to the 62.7–63 mol% reported for P. flavescens but slightly higher than the 62.5–62.6 mol% of P. straminea and significantly higher than the 57.5–58.0 mol% of P. argentinensis. The major cellular fatty acids present in the CA5T strain were C18:1ω7c (41.1%), C16:1ω6c and C16:1ω7c (25.7%), C16:0 (12.0%), C12:0 (8.0%), C12:0 3-OH (4.4%), and C10:0 3-OH (2.9%). The cellular fatty acid profile, GC content, phenotypic properties, and biochemical characteristics were consistent with its placement within the genus Pseudomonas. The name P. seleniipraecipitatus is proposed for these bacteria.