Fewer essential genes in mycoplasmas than previous studies suggest
Fewer essential genes in mycoplasmas than previous studies suggest
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DOI：
10.1111/j.1574-6968.2010.02078.x
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发表时间：
2010-10-01
影响因子：
2.1
通讯作者：
Simmons, Warren L.
中科院分区：
文献类型：
--
作者：
Dybvig, Kevin;Lao, Ping;Simmons, Warren L.
在这里，我们描述了肺支原体的突变体，获得使用minitransposon，Tn4001TF1，积极转座，但随后无法进行后续切除事件。使用Tn4001TF1，我们破坏了39个以前被认为对生长至关重要的基因。因此，生长所需的基因数量被高估了。本研究还揭示了M. pummonis的染色体分离蛋白，并鉴定了在支原体中存在但在枯草芽孢杆菌中必需的染色体分离蛋白。
Here, we describe mutants of Mycoplasma pulmonis that were obtained using a minitransposon, Tn4001TF1, which actively transposes but is then unable to undergo subsequent excision events. Using Tn4001TF1, we disrupted 39 genes previously thought to be essential for growth. Thus, the number of genes required for growth has been overestimated. This study also revealed evidence of gene duplications in M. pulmonis and identified chromosome segregation proteins that are dispensable in mycoplasmas but essential in Bacillus subtilis.