AN ANALYSIS OF THE ORIGINS OF THE CHOLINERGIC AND NONCHOLINERGIC SEPTAL PROJECTIONS TO THE HIPPOCAMPAL-FORMATION OF THE RAT
AN ANALYSIS OF THE ORIGINS OF THE CHOLINERGIC AND NONCHOLINERGIC SEPTAL PROJECTIONS TO THE HIPPOCAMPAL-FORMATION OF THE RAT
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DOI：
10.1002/cne.902400104
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发表时间：
1985-01-01
影响因子：
2.5
通讯作者：
KURZ, J
中科院分区：
文献类型：
--
作者：
AMARAL, DG;KURZ, J
这些实验旨在确定投射到大鼠海马结构的胆碱能隔细胞的比例和分布。将WGA-HRP注射到海马结构的不同区域，并通过隔区复合体处理切片，以同时显示逆行运输的标记和胆碱乙酰转移酶(ChAT)免疫反应。对显示ChAT或Nissl染色的相邻正常序列的初步分析显示，在经典定义的内侧隔核和斜角带核的垂直支中有几个不同的细胞群。细胞群的范围从几乎完全ChAT阳性到完全非胆碱能。根据构成细胞的形状和大小，间隔复合体的ChAT阳性细胞被分为背侧、中间和腹侧亚群。在不同的实验中，逆行标记细胞也呈ChAT阳性的比例从22.8%到77.4%不等。当只考虑海马区和齿状回时，这种差异很大程度上可以由隔-海马区投射的地形组织来解释。内侧隔核的内侧非胆碱能半部分主要投射到齿状回和海马区的吻侧或隔部，而背侧聊天组所在的外侧半侧主要投射到更多的颞叶水平。海马头侧部和齿状回的大部分胆碱能输入来自腹侧Chat细胞群，后者构成斜角带核垂直支的主要组成部分。虽然中间组中的一些ChAT阳性细胞投射到海马结构，但它们的数量通常比背侧组和腹侧组的细胞少。然而，在一项对照实验中，将WGA-HRP注射到位于海马嘴结构上方的扣带回皮质，中间ChAT组占双标细胞的71.2%。
These experiments were directed at determining the proportion and distribution of cholinergic septal cells which project to the rat hippocampal formation. Injections of WGA-HRP were placed into different regions of the hippocampal formation and sections through the septal complex were processed for the simultaneous demonstration of the retrogradely transported marker and for choline acetyltransferase (ChAT) immunoreactivity. Preliminary analysis of adjacent normal series prepared either for the demonstration of ChAT or stained by the Nissl method demonstrated several distinct cell groups in the classically defined medial septal nucleus and vertical limb of the nucleus of the diagonal band. The groups of cells ranged from almost entirely ChAT-positive to entirely noncholinergic. On the basis of shape and size of the constituent cells, the ChAT-positive cells of the septal complex were divided into dorsal, intermediate, and ventral subdivisions. The proportion of retrogradely labeled cells that were also ChAT positive ranged from 22.8% to 77.4% in different experiments. When only the hippocampus and dentate gyrus are considered, this variation can largely be accounted for by the topographic organization of the septohippocampal projection. The medial, noncholinergic half of the medial septal nucleus projects primarily to the rostral or septal portions of the dentate gyrus and hippocampus, whereas the lateral half, in which the dorsal ChAT group is located, projects heavily to more temporal levels. Rostral portions of the hippocampus and dentate gyrus receive most of their cholinergic input from the ventral ChAT cell group which forms a major component of the vertical limb of the nucleus of the diagonal band. While some ChAT-positive cells in the intermediate group project to the hippocampal formation, they are generally less numerous than those from the dorsal and ventral groups. However, in a control experiment in which the WGA-HRP injection was placed into the cingulate cortex overlying the rostral hippocampal formation, the intermediate ChAT group accounted for 71.2% of the double-labeled cells.