Label-Free Biosensor Imaging on Photonic Crystal Surfaces.
Label-Free Biosensor Imaging on Photonic Crystal Surfaces.
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DOI：
10.3390/s150921613
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发表时间：
2015-08-28
期刊：
影响因子：
--
通讯作者：
Cunningham BT
中科院分区：
文献类型：
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作者：
Zhuo Y;Cunningham BT
我们回顾了纳米结构光子晶体表面和高光谱反射成像检测仪器的发展和应用，当它们一起使用时，代表了一种新形式的光学显微镜，可以无标记，定量和动力学监测生物材料与基底表面的相互作用。光子晶体增强显微镜（PCEM）已被用于检测广泛类别的材料，包括介电纳米颗粒、金属等离子体纳米颗粒、生物分子层和活细胞。由于PCEM不需要细胞毒性染料或可光漂白的荧光染料，因此它特别适用于监测细胞与细胞外基质表面的长期相互作用。PCEM仅对光子晶体表面~200 nm内的细胞组分的附着敏感，这可能对应于涉及干细胞分化、趋化性和转移的粘附过程的最感兴趣的区域。PCEM还证明了足够的灵敏度，用于感测与蛋白质分子大小大致相同的纳米颗粒造影剂，这可以实现在具有单分子感测分辨率的“数字”诊断中的应用。我们将回顾PCEM的发展历史，工作原理，纳米结构设计和成像模式，使光学散射体，发射器，吸收器和介电常数中心的跟踪。
We review the development and application of nanostructured photonic crystal surfaces and a hyperspectral reflectance imaging detection instrument which, when used together, represent a new form of optical microscopy that enables label-free, quantitative, and kinetic monitoring of biomaterial interaction with substrate surfaces. Photonic Crystal Enhanced Microscopy (PCEM) has been used to detect broad classes of materials which include dielectric nanoparticles, metal plasmonic nanoparticles, biomolecular layers, and live cells. Because PCEM does not require cytotoxic stains or photobleachable fluorescent dyes, it is especially useful for monitoring the long-term interactions of cells with extracellular matrix surfaces. PCEM is only sensitive to the attachment of cell components within ~200 nm of the photonic crystal surface, which may correspond to the region of most interest for adhesion processes that involve stem cell differentiation, chemotaxis, and metastasis. PCEM has also demonstrated sufficient sensitivity for sensing nanoparticle contrast agents that are roughly the same size as protein molecules, which may enable applications in “digital” diagnostics with single molecule sensing resolution. We will review PCEM’s development history, operating principles, nanostructure design, and imaging modalities that enable tracking of optical scatterers, emitters, absorbers, and centers of dielectric permittivity.