PorA, a conserved C-terminal domain-containing protein, impacts the PorXY-SigP signaling of the type IX secretion system.
PorA, a conserved C-terminal domain-containing protein, impacts the PorXY-SigP signaling of the type IX secretion system.
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DOI：
10.1038/s41598-020-77987-y
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发表时间：
2020-12-03
影响因子：
4.6
通讯作者：
Nakayama K
中科院分区：
文献类型：
--
作者：
Yukitake H;Shoji M;Sato K;Handa Y;Naito M;Imada K;Nakayama K
牙龈卟啉单胞菌（Porphyromonas gingivalis）是一种牙周病病原体，其通过IX型分泌系统（T9 SS）将包括半胱氨酸蛋白酶（称为牙龈卟啉菌蛋白酶）在内的许多毒力因子易位到细胞表面。T9 SS组分蛋白的表达受PorXY双组分系统和ECF sigma因子SigP的串联信号传导调节。然而，这种调控途径的细节仍然未知。我们发现，T9 SS保守的C-末端结构域包含的蛋白质，PGN_0123，我们已指定PorA，是参与调节编码T9 SS结构蛋白的基因的表达，PorA可以易位到细胞表面上没有T9 SS易位机制。X-射线晶体学分析表明，PorA具有类似于大肠杆菌FimH的甘露糖结合结构域的结构域，FimH是1型菌毛的尖端蛋白。传感激酶PorY胞质结构域的突变赋予ΔporA突变体表型恢复。在ΔporA突变体中，由PorXY双组分系统激活的SigP sigma因子显着减少。这些结果强烈支持PorA在将信号从细胞表面传递到PorXY-SigP信号传导途径中的潜在作用。
Porphyromonas gingivalis, a periodontal pathogen, translocates many virulence factors including the cysteine proteases referred to as gingipains to the cell surface via the type IX secretion system (T9SS). Expression of the T9SS component proteins is regulated by the tandem signaling of the PorXY two-component system and the ECF sigma factor SigP. However, the details of this regulatory pathway are still unknown. We found that one of the T9SS conserved C-terminal domain-containing proteins, PGN_0123, which we have designated PorA, is involved in regulating expression of genes encoding T9SS structural proteins and that PorA can be translocated onto the cell surface without the T9SS translocation machinery. X-ray crystallography revealed that PorA has a domain similar to the mannose-binding domain of Escherichia coli FimH, the tip protein of Type 1 pilus. Mutations in the cytoplasmic domain of the sensor kinase PorY conferred phenotypic recovery on the ΔporA mutant. The SigP sigma factor, which is activated by the PorXY two-component system, markedly decreased in the ΔporA mutant. These results strongly support a potential role for PorA in relaying a signal from the cell surface to the PorXY-SigP signaling pathway.
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影响因子：
4.6
作者：
Kadowaki T;Yukitake H;Naito M;Sato K;Kikuchi Y;Kondo Y;Shoji M;Nakayama K
通讯作者：
Nakayama K
影响因子：
3.1
作者：
Kondo, Yoshio;Ohara, Naoya;Nakayama, Koji
通讯作者：
Nakayama, Koji
影响因子：
3.7
作者：
Dou, Y.;Aruni, W.;Fletcher, H. M.
通讯作者：
Fletcher, H. M.
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH
影响因子：
6.7
作者：
Gorasia DG;Veith PD;Chen D;Seers CA;Mitchell HA;Chen YY;Glew MD;Dashper SG;Reynolds EC
通讯作者：
Reynolds EC