Single-dose, virus-vectored vaccine protection against Yersinia pestis challenge: CD4+ cells are required at the time of challenge for optimal protection
Single-dose, virus-vectored vaccine protection against Yersinia pestis challenge: CD4+ cells are required at the time of challenge for optimal protection
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DOI：
10.1016/j.vaccine.2008.09.031
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发表时间：
2008-11-25
期刊：
影响因子：
5.5
通讯作者：
Rose, John K.
中科院分区：
文献类型：
--
作者：
Chattopadhyay, Anasuya;Park, Steven;Rose, John K.
我们已经开发了一种实验性的重组水泡性口炎病毒（VSV）载体鼠疫疫苗表达的分泌形式的鼠疫耶尔森氏菌低钙反应蛋白V（LcrV）从VSV基因组的第一个位置。这种载体，在单剂量肌肉注射，诱导高水平的抗体滴度LcrV，并提供90-100%的保护，对肺鼠疫攻击小鼠。这种单剂量保护显著优于表达非分泌型LcrV蛋白的VSV产生的保护。增加的保护与增加的抗LcrV抗体和偏向IgG 2a和远离IgG 1同种型。我们还发现，在攻击时，CD 4（+）细胞而不是CD 8（+）细胞的耗竭导致疫苗保护作用降低，表明细胞免疫在保护中的作用。(c)2008爱思唯尔有限公司保留所有权利。
We have developed an experimental recombinant vesicular stomatitis virus (VSV) vectored plague vaccine expressing a secreted form of Yersinia pestis low calcium response protein V (LcrV) from the first position of the VSV genome. This vector, given intramuscularly in a single dose, induced high-level antibody titers to LcrV and gave 90-100% protection against pneumonic plague challenge in mice. This single-dose protection was significantly better than that generated by VSV expressing the non-secreted LcrV protein. Increased protection correlated with increased anti-LcrV antibody and a bias toward IgG2a and away from IgG1 isotypes. We also found that the depletion of CD4(+) cells, but not CD8(+) cells, at the time of challenge resulted in reduced vaccine protection, indicating a role for cellular immunity in protection. (c) 2008 Elsevier Ltd. All rights reserved.