Genome characteristics reveal the impact of lichenization on lichen-forming fungus Endocarpon pusillum Hedwig (Verrucariales, Ascomycota).
Genome characteristics reveal the impact of lichenization on lichen-forming fungus Endocarpon pusillum Hedwig (Verrucariales, Ascomycota).
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基因组特征揭示了地衣化对地衣形成真菌 Endocarpon pusillum Hedwig（疣孢菌目，子囊菌门）的影响
DOI：
10.1186/1471-2164-15-34
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发表时间：
2014-01-17
期刊：
影响因子：
4.4
通讯作者：
Wei JC
中科院分区：
文献类型：
--
作者：
Wang YY;Liu B;Zhang XY;Zhou QM;Zhang T;Li H;Yu YF;Zhang XL;Hao XY;Wang M;Wang L;Wei JC
地衣是一种典型的互惠共生生物，地衣化是真菌共生的一种。地衣形成菌Endocarpon pusillum是干旱地区与绿色的双球腔菌Diplosphaera chodatii Bialsuknia共生的地衣。pusillum。在37.5Mb的E. pusillum。对基因的分析为某些自然特征提供了直接的分子证据，如同宗配合生殖和耐旱性。比较基因组学分析表明，大肠杆菌中某些蛋白质家族的扩增和收缩与大肠杆菌中某些蛋白质家族的扩增和收缩有关。pusillum基因组反映了其与光合伴侣（D. chodatii）。利用地衣形成真菌E. pusillum和它的藻类伴侣允许参与共生体之间的氮和碳转移的基因的功能鉴定，并且发现三种不含信号肽结构域的凝集素对于地衣中的共生识别是必需的;有趣是，两种地衣的生物量之比形成真菌及其光合伙伴和他们的接触时间被发现是重要的，这两个共生体之间的相互作用。地衣形成真菌E. pusillum的一般生物学特性以及该菌与其光合作用伙伴D.为探讨其抗旱性和共生性的本质提供了研究依据。
BackgroundLichen is a classic mutualistic organism and the lichenization is one of the fungal symbioses. The lichen-forming fungus Endocarpon pusillum is living in symbiosis with the green alga Diplosphaera chodatii Bialsuknia as a lichen in the arid regions.Results454 and Illumina technologies were used to sequence the genome of E. pusillum. A total of 9,285 genes were annotated in the 37.5 Mb genome of E. pusillum. Analyses of the genes provided direct molecular evidence for certain natural characteristics, such as homothallic reproduction and drought-tolerance. Comparative genomics analysis indicated that the expansion and contraction of some protein families in the E. pusillum genome reflect the specific relationship with its photosynthetic partner (D. chodatii). Co-culture experiments using the lichen-forming fungus E. pusillum and its algal partner allowed the functional identification of genes involved in the nitrogen and carbon transfer between both symbionts, and three lectins without signal peptide domains were found to be essential for the symbiotic recognition in the lichen; interestingly, the ratio of the biomass of both lichen-forming fungus and its photosynthetic partner and their contact time were found to be important for the interaction between these two symbionts.ConclusionsThe present study lays a genomic analysis of the lichen-forming fungus E. pusillum for demonstrating its general biological features and the traits of the interaction between this fungus and its photosynthetic partner D. chodatii, and will provide research basis for investigating the nature of its drought resistance and symbiosis.
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影响因子：
4.4
作者：
Fedorova ND;Badger JH;Robson GD;Wortman JR;Nierman WC
通讯作者：
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