Development of powdery mildew resistant derivatives of wheat variety Fielder for use in genetic transformation
Development of powdery mildew resistant derivatives of wheat variety Fielder for use in genetic transformation
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DOI：
10.1016/j.cj.2022.06.012
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发表时间：
2023-04-01
期刊：
影响因子：
6.6
通讯作者：
Ye, Xingguo
中科院分区：
文献类型：
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作者：
Liang, Xiaona;Bie, Xiaomin;Ye, Xingguo
遗传转化广泛用于改善小麦的目标性状和研究基因功能。然而，转化效率取决于受体基因型的生理状态，并受到包括白粉病 (PM) 感染在内的多种因素的影响。广泛使用的受体品种 Fielder 非常容易受到 PM 的影响。因此，开发具有高再生能力的PM抗性衍生物用于遗传转化将是有益的。在本研究中，分别携带基因Pm21和PmV的PM抗性品系CB037和Pm97033与Fielder回交，并选择PM抗性。培育了带有Pm21的NT89、NT90、NT154和WT48以及带有PmV的FL347等5个品系，通过分子标记和基因组原位杂交（GISH）或荧光原位杂交（FISH）进行鉴定，并进一步对农艺性状和遗传转化后的再生能力进行了详细评估。经评估，在再生和转化能力方面与Fielder相当或优于Fielder的品系FL347、WT48、NT89和NT154被推荐作为替代Fielder用于小麦基因转移和基因组编辑研究的合适材料。 (c) 2022 中国作物学会、中国农业科学院作物科学研究所。由 Elsevier B.V. 代表科爱通讯有限公司制作和托管。这是一篇基于 CC BY-NC-ND 许可 (http://creativecommons.org/licenses/by-nc-nd/4.0/) 的开放获取文章。
Genetic transformation is widely used to improve target traits and to study gene function in wheat. However, transformation efficiency depends on the physiological status of the recipient genotype and that is affected by several factors including powdery mildew (PM) infection. The widely used recipient variety Fielder is very susceptible to PM. Therefore, it would be beneficial to develop PM resistant deriva-tives with high regeneration ability for use in genetic transformation. In the present study PM resistant lines CB037 and Pm97033 carrying genes Pm21 and PmV, respectively, were backcrossed to Fielder with selection for PM resistance. Five lines, NT89, NT90, NT154, and WT48 with Pm21 and line FL347 with PmV were developed, identified by molecular markers and genomic in situ hybridization (GISH) or fluorescent in situ hybridization (FISH), and further subjected to detailed assessment of agronomic traits and regen-eration ability following genetic transformation capacity. Lines FL347, WT48, NT89 and NT154 assessed as being equal to, or superior, to Fielder in regeneration and transformation ability are recommended as suitable materials for the replacement of Fielder for wheat gene transfer and genome editing study. (c) 2022 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).