Rapid inactivation of airborne porcine reproductive and respiratory syndrome virus using an atmospheric pressure air plasma
Rapid inactivation of airborne porcine reproductive and respiratory syndrome virus using an atmospheric pressure air plasma
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DOI：
10.1002/ppap.201900269
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发表时间：
2020-02
影响因子：
3.5
通讯作者：
G. Nayak;Austin J. Andrews;I. Marabella;H. Aboubakr;S. Goyal;B. Olson;M. Torremorell;P. Bruggeman-P.-Bru
中科院分区：
文献类型：
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作者：
G. Nayak;Austin J. Andrews;I. Marabella;H. Aboubakr;S. Goyal;B. Olson;M. Torremorell;P. Bruggeman-P.-Bru
空气传播疾病在动物中的传播对动物安全构成巨大风险，并可能造成重大经济损失。在这项研究中，我们报告了使用介质阻挡放电（DBD）在空中灭活空气中雾化的猪生殖与呼吸综合征病毒。与DBD反应器上游相比，通过TCID50方法测定的下游采样病毒的感染性显示病毒滴度降低了约3.5 log10。通过逆转录定量真实的时间聚合酶链反应方法对病毒基因组进行的独立检测证实了在最小过滤效应下的灭活。认为载病毒液滴中的短寿命物质（如和）和低pH值下的过氧亚硝酸（ONOOH）化学是观察到的灭活的原因。
The transmission of airborne diseases in animals poses great risks to animal safety with potential significant economic losses. In this study, we report on the use of a dielectric barrier discharge (DBD) for in‐flight inactivation of an airborne aerosolized porcine reproductive and respiratory syndrome virus. The infectivity of the sampled virus downstream compared to upstream of the DBD reactor as determined by the TCID50method showed a ∼3.5 log10reduction in the virus titer. Independent testing of the viral genome by the reverse‐transcription quantitative real‐time polymerase chain reaction method confirmed the inactivation with minimal filtering effects. Both short‐lived species such as and and peroxynitrous acid (ONOOH) chemistry at low pH in the virus‐laden droplets are suggested to be responsible for the observed inactivation.