On the use of DHB/aniline and DHB/N,N-dimethylaniline matrices for improved detection of carbohydrates:: Automated identification of oligosaccharides and quantitative analysis of sialylated glycans by MALDI-TOF mass spectrometry
On the use of DHB/aniline and DHB/N,N-dimethylaniline matrices for improved detection of carbohydrates:: Automated identification of oligosaccharides and quantitative analysis of sialylated glycans by MALDI-TOF mass spectrometry
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DOI：
10.1016/j.jasms.2008.04.033
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发表时间：
2008-08-01
影响因子：
3.2
通讯作者：
Perreault, Helene
中科院分区：
文献类型：
--
作者：
Snovida, Sergei I.;Rak-Banville, Justin M.;Perreault, Helene
本研究展示了2,5-二羟基苯甲酸/苯胺（DHB/An）和2,5-二羟基苯甲酸/N，N-二甲苯胺（DHB/DMA）基质辅助激光解吸/电离质谱（MALDI-MS）对天然低聚糖的自动鉴定和定量分析的应用。在分析物的信号强度和整个晶体层样品分布的均匀性方面，两种基质均优于纯DHB。DHB/An中稳定的苯胺希夫碱聚糖衍生物的靶形成以及DHB/DMA矩阵中获得的质谱中完全没有此类产物，为复杂样品MALDI质谱中的还原性低聚糖的自动鉴定提供了平台。该研究还展示了如何使用这些基质来提高灵敏度，以及如何利用沉积样品材料的均匀性，在低纳克到中皮克范围内快速准确地定量分析含有中性和唾液化低聚糖的天然聚糖混合物。
This study demonstrates the application of 2,5-dihydrohybenzoic acid/aniline (DHB/An) and 2,5-dihydroxybenzoic acid/N,N-dimethylaniline (DHB/DMA) matrices for automated identification and quantitative analysis of native oligosaccharides by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). Both matrices are shown to be superior to pure DHB for native glycans in terms of signal intensities of analytes and homogeneity of sample distribution throughout the crystal layer. On-target formation of stable aniline Schiff base derivatives of glycans in DHB/An and the complete absence of such products in the mass spectra acquired in DHB/DMA matrix provide a platform for automated identification of reducing oligosaccharides in the MALDI mass spectra of complex samples. The study also shows how enhanced sensitivity is achieved with the use of these matrices and how the homogeneity of deposited sample material may be exploited for quick and accurate quantitative analysis of native glycan mixtures containing neutral and sialylated oligosaccharides in the low-nanogram to mid-picogram range.