The Role of Beta-Adrenergic Receptors in the Regulation of Circadian Intraocular Pressure Rhythm in Mice.
The Role of Beta-Adrenergic Receptors in the Regulation of Circadian Intraocular Pressure Rhythm in Mice.
复制标题
β-肾上腺素能受体在小鼠昼夜眼压节律调节中的作用。
DOI：
10.1080/02713683.2016.1264605
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发表时间：
2017
影响因子：
2
通讯作者：
Sugiyama K.
中科院分区：
文献类型：
--
作者：
Tsuchiya S;Higashide T;Toida K;Sugiyama K.
目的：探讨β1-和β2肾上腺素能受体的消除是否会改变小鼠的昼夜眼压节律。材料与方法：对β1-/β2肾上腺素能受体双敲除小鼠和C57BL/6J小鼠进行腹腔麻醉，微针法测定其IOPs。进入12 h光暗（LD）周期（光期6:00-18:00）后，从9:00至24:00每3 h测量一次IOPs（第1组，β1-/β2肾上腺素能受体双敲除小鼠，n= 11; C57BL/6J,n= 15）。另一组β1-/β2肾上腺素受体双敲除小鼠（组2，n= 12）分别在12 h LD周期下15:00和24:00和恒定黑暗（分别在黑暗暴露后1天和8天）进行IOP测量。使用线性混合模型评估全天IOP方差和时间点之间的平均IOP差异。结果：β1-/β2肾上腺素能受体双敲除小鼠和C57BL/6J小鼠的IOP曲线呈双相变化，在光照期低，在黑暗期高；波动显著（P< 0.001）。β1-/β2肾上腺素能受体双敲除小鼠眼压峰值（18.7±1.4 mmHg）出现在24:00，眼压谷（13.5±1.5 mmHg）出现在15:00。β1-/β2-肾上腺素能受体双敲除小鼠的IOP曲线与C57BL/6J几乎平行，且β1-/β2-肾上腺素能受体双敲除小鼠的IOP显著高于C57BL/6J小鼠（P< 0.001）。在持续黑暗（DD）条件下，24时（18.1±1.5 mmHg）的IOP显著高于15时（13.3±1.2 mmHg） （P< 0.001）。从LD周期到DD环境的转变对IOP没有显著影响（P= 0.728）。结论：消除β1-和β2-肾上腺素能受体不会干扰小鼠双相眼压昼夜节律。
Purpose: To investigate whether the elimination of β1- and β2-adrenergic receptors alters the diurnal intraocular pressure (IOP) rhythm in mice.Materials and Methods: β1-/β2-adrenergic receptor double-knockout and C57BL/6J mice were anesthetized intraperitoneally, with their IOPs measured via microneedle method. After entrainment to a 12-h light–dark (LD) cycle (light phase 6:00–18:00), IOPs were measured every 3 h from 9:00 to 24:00 (group 1, β1-/β2-adrenergic receptor double-knockout mice,n= 11; C57BL/6J,n= 15). The IOP measurements at 15:00 and 24:00 under a 12-h LD cycle and in the constant darkness (1 day and 8 days after exposure to darkness, respectively) were performed in another group of β1-/β2-adrenergic receptor double-knockout mice (group 2,n= 12). IOP variance throughout the day and mean IOP differences among time points were evaluated using a linear mixed model.Results: β1-/β2-adrenergic receptor double-knockout and C57BL/6J mice showed biphasic IOP curves, low during the light phase and high during the dark phase; the fluctuation was significant (P< 0.001). The peak IOP (18.7 ± 1.4 mmHg) occurred at 24:00 and the trough IOP (13.5 ± 1.5 mmHg) occurred at 15:00 in β1-/β2-adrenergic receptor double-knockout mice group. IOP curves of β1-/β2-adrenergic receptor double-knockout and C57BL/6J were nearly parallel, and the IOPs of β1-/β2-adrenergic receptor double-knockout mice were significantly higher than those of C57BL/6J mice (P< 0.001). Under constant dark (DD) conditions, IOP at 24:00 (18.1 ± 1.5 mmHg) was significantly higher than that at 15:00 (13.3 ± 1.2 mmHg) (P< 0.001). The transition from the LD cycle to DD environment produced no significant change in IOP (P= 0.728).Conclusions: Elimination of both β1- and β2-adrenergic receptors did not disturb the biphasic diurnal IOP rhythm in mice.