Polarized expression of members of the solute carrier SLC19A gene family of water-soluble multivitamin transporters:: implications for physiological function
Polarized expression of members of the solute carrier SLC19A gene family of water-soluble multivitamin transporters:: implications for physiological function
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DOI：
10.1042/bj20031220
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发表时间：
2003-11-15
影响因子：
4.1
通讯作者：
Marchant, JS
中科院分区：
文献类型：
--
作者：
Boulware, MJ;Subramanian, VS;Marchant, JS
人类缺乏合成微量营养素硫胺素和叶酸的生化途径。细胞需要通过膜转运活性来满足，这是由SLC19A基因家族蛋白介导的。通过使用活细胞共聚焦成像方法解析所有SLC19A家族成员的定位，我们发现这两种人类硫胺转运蛋白在极化细胞中存在差异靶向，建立了载体转运系统。这种极化减少了转运体异构体之间的功能冗余，并允许细胞中硫胺素进口和出口途径的独立调节。
Humans lack biochemical pathways for the synthesis of the micro-nutrients thiamine and folate. Cellular requirements are met through membrane transport activity, which is mediated by proteins of the SLC19A gene family. By using live-cell confocal imaging methods to resolve the localization of all SLC19A family members, we show that the two human thiamine transporters are differentially targeted in polarized cells, establishing a vectorial transport system. Such polarization decreases functional redundancy between transporter isoforms and allows for independent regulation of thiamine import and export pathways in cells.