Interaction of enterohemorrhagic Escherichia coli O157:H7 with mouse intestinal mucosa
Interaction of enterohemorrhagic Escherichia coli O157:H7 with mouse intestinal mucosa
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DOI：
10.1111/j.1574-6968.2008.01166.x
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发表时间：
2008-06-01
影响因子：
2.1
通讯作者：
La Ragione, Roberto M.
中科院分区：
文献类型：
--
作者：
Girard, Francis;Frankel, Gad;La Ragione, Roberto M.
在本研究中，我们使用小鼠回肠环来研究肠出血性大肠杆菌 (EHEC) O157:H7 与小鼠肠粘膜的相互作用。在剂量为 10(9) 并孵育 3 小时的情况下，在管腔中检测到 EHEC O157，并且在较小程度上与上皮相关。可以看到典型的附着和抹去（A/E）损伤，尽管这种情况很少见。虽然效应蛋白 Tir 对于 A/E 病变形成至关重要，但细菌 III 型分泌系统接头蛋白 TccP 是可有可无的。这些结果表明，小鼠肠粘膜上的 A/E 损伤可以独立于强肌动蛋白聚合而形成。
In this study, we used mouse ileal loops to investigate the interaction of enterohemorrhagic Escherichia coli (EHEC) O157:H7 with the mouse intestinal mucosa. With a dose of 10(9) and 3 h incubation, EHEC O157 was detected in the lumen and to a lesser extent associated with the epithelium. Typical attaching and effacing (A/E) lesions were seen, albeit infrequently. While the effector protein Tir was essential for A/E lesion formation, the bacterial type III secretion system adaptor protein TccP was dispensable. These results suggest that A/E lesions on mouse intestinal mucosa can be formed independently of robust actin polymerization.