Localization of endothelin-like immunoreactivity in rat kidneys.
Localization of endothelin-like immunoreactivity in rat kidneys.
复制标题
大鼠肾脏内皮素样免疫反应性的定位。
DOI：
10.1152/ajprenal.1991.260.6.f913
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Nord,EP
中科院分区：
文献类型：
--
作者：
Wilkes,BM;Susin,M;Mento,PF;Macica,CM;Girardi,EP;Boss,E;Nord,EP
本文用免疫过氧化物酶技术观察了内皮素样免疫反应阳性物质在正常大鼠肾脏的分布。在家兔体内制备了内皮素1的特异性多克隆抗体，该抗体可识别内皮素1及其前体分子大内皮素。免疫过氧化物酶染色显示，在肾皮质，髓质和乳头特异性内皮素样免疫反应。免疫染色密度在肾乳头中最大，其中染色主要定位于远端肾单位段的直小血管。在肾乳头的集合管细胞中观察到局灶性细胞质免疫染色。在肾髓质中，在直血管中发现了强烈的免疫染色。皮质免疫染色定位于弓形动脉、静脉、小动脉和管周毛细血管的内皮表面。肾小球免疫染色遵循毛细血管袢分布，似乎主要局限于内皮细胞，少量反应产物覆盖系膜。近端小管刷状缘和乳头状集合管上皮的最近端部分显示局灶性内皮素样免疫染色。我们的结论是内皮素样免疫反应广泛分布在肾脏组织中的重要自分泌和旁分泌行动兼容。
The distribution of endothelin-like immunoreactivity was examined in normal rat kidneys using the immunoperoxidase technique. A specific polyclonal antibody to endothelin 1, which recognized endothelin 1 and its precursor molecule, big endothelin, was raised in rabbits. Immunoperoxidase staining revealed specific endothelin-like immunoreactivity in the renal cortex, medulla, and papilla. Immunostaining density was greatest in the renal papilla where staining was predominantly localized to the vasa rectae of the distal nephron segments. Cytoplasmic immunostaining was noted focally in collecting duct cells in the renal papilla. In the renal medulla, intense immunostaining was identified in the vasa rectae. Cortical immunostaining was localized to the endothelial surfaces of arcuate arteries, veins, arterioles and peritubular capillaries. Glomerular immunostaining followed a capillary loop distribution and appeared to be predominantly localized to endothelial cells with smaller amounts of reaction product overlying the mesangium. The most proximal portion of the proximal tubule brush border and papillary collecting duct epithelium demonstrated focal endothelin-like immunostaining. We conclude that endothelin-like immunoreactivity is widely distributed in renal tissue compatible with important autacrine and paracrine actions in the kidneys.