Strontium and magnesium ions released from bioactive titanium metal promote early bone bonding in a rabbit implant model
Strontium and magnesium ions released from bioactive titanium metal promote early bone bonding in a rabbit implant model
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DOI：
10.1016/j.actbio.2017.09.019
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发表时间：
2017-11-01
影响因子：
9.7
通讯作者：
Matsuda, Shuichi
中科院分区：
文献类型：
--
作者：
Okuzu, Yaichiro;Fujibayashi, Shunsuke;Matsuda, Shuichi
我们以前开发了“碱和热处理”方法，以赋予钛金属（Ti）生物活性（骨结合能力）。据报道，锶（Sr）和镁（Mg）离子促进成骨细胞增殖和分化，并加速骨形成，我们改进了这种方法，以诱导释放Sr（Sr-Ti）或Mg（Mg-Ti）离子从钛在以前的研究。在这里，我们评估了这些新的表面处理，Sr-Ti和Mg-Ti的生物活性。使用MC 3 T3-E1细胞对细胞活力、整联蛋白#1、β连环蛋白和细胞周期蛋白D1的表达、成骨基因表达、碱性磷酸酶活性和细胞外矿化进行的体外评价显示Sr-Ti和Mg-Ti增强增殖和成骨分化。在家兔体内研究中，Sr-Ti和Mg-Ti也提供了比阳性对照Ti（Ca-Ti）更大的生物力学强度和骨-植入物接触，尤其是在早期（4-8周），并在更长时间内（16-24周）保持这些特性。改进的方法的优点包括工艺简单、适用于任何植入物形状以及对植入物组成和结构没有不利影响。因此，我们的治疗是有希望的临床应用，以实现早期骨bonding.Statement的显著性植入到骨质疏松的骨构成了一个具有挑战性的问题，因为早期迁移或松动的植入物，这主要是由于不充分的初始固定在多孔骨。因此，希望提供具有早期骨结合能力的植入物。我们已经实现了通过释放锶离子或镁离子赋予钛金属早期骨结合能力。我们的治疗是有希望的临床应用，以实现骨科或牙科钛基植入物的早期骨结合。(C)2017 Acta Materialia Inc.由爱思唯尔有限公司出版。保留所有权利。
We have previously developed the "alkali and heat treatment" method to confer bioactivity (bone bonding ability) to titanium metal (Ti). As strontium (Sr) and magnesium (Mg) ions reportedly promote osteoblastic cell proliferation and differentiation and accelerate bone formation, we improved this method to induce the release of Sr (Sr-Ti) or Mg (Mg-Ti) ions from Ti in a previous study. Here, we evaluated the bioactivity of these novel surface treatments, Sr-Ti and Mg-Ti. In vitro evaluation of cell viability, expression of integrin #1, # catenin, and cyclin Dl, osteogenic gene expression, alkaline phosphatase activity, and extracellular mineralization using MC3T3-E1 cells revealed that Sr-Ti and Mg-Ti enhanced proliferation and osteogenic differentiation. In rabbit in vivo studies, Sr-Ti and Mg-Ti also provided greater biomechanical strength and bone-implant contact than the positive control Ti (Ca-Ti), especially at the early stage (4-8 weeks), and maintained these properties for a longer period (16-24 weeks). Advantages of the improved method include process simplicity, applicability for any implant shape, and lack of adverse effects on implant composition and structure. Therefore, our treatment is promising for clinical applications to achieve early bone bonding.Statement of SignificanceImplantation into osteoporotic bone constitutes a challenging problem because of early migration or loosening of the implant, which is primarily due to insufficient initial fixation in porotic bone. Therefore, it is desirable to provide implants with a capacity for early bone bonding. We have achieved conferring early bone bonding ability to titanium metal by releasing strontium ions or magnesium ions. Our treatment is promising for clinical applications to achieve early bone bonding of orthopedic or dental Ti-based implants. (C) 2017 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.