Near-Infrared Fluorescent Turn-on Probe with a Remarkable Large Stokes Shift for Imaging Selenocysteine in Living Cells and Animals
Near-Infrared Fluorescent Turn-on Probe with a Remarkable Large Stokes Shift for Imaging Selenocysteine in Living Cells and Animals
复制标题
具有显着大斯托克斯位移的近红外荧光开启探针，用于活细胞和动物中的硒代半胱氨酸成像
DOI：
10.1021/acs.analchem.7b00824
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发表时间：
2017-06-06
影响因子：
7.4
通讯作者：
Feng, Guoqiang
中科院分区：
文献类型：
--
作者：
Feng, Weiyong;Li, Meixing;Feng, Guoqiang
硒代半胱氨酸（Sec）是第21种天然存在的氨基酸，近年来已成为一个重要的检测靶点。然而，在生物系统中对Sec进行荧光检测具有挑战性。到目前为止，报道的荧光Sec探针非常少，且大多数在可见光区域对Sec产生荧光响应。在本文中，开发了一种非常有前景的用于Sec的近红外荧光探针。该探针在温和条件下的宽pH范围内的水溶液中起作用，可用于对Sec进行快速、高选择性和高灵敏度的检测，并伴有显著的近红外荧光增强信号变化。此外，它具有显著的大斯托克斯位移（192纳米），对Sec的检测限低（60纳摩尔），线性范围宽（0 - 70微摩尔）。而且，该探针可方便地用于检测血清样本、活细胞和动物中的Sec，这表明它在生物应用方面具有很大的潜力。
Selenocysteine (Sec) is the 21st naturally occurring amino acid and has emerged as an important sensing target in recent years. However, fluorescent detection of Sec in living systems is challenging. To date, very few fluorescent Sec probes have been reported and most of them respond fluorescence to Sec in the-visible region. In this paper, a very promising near-infrared fluorescent probe for Sec was developed. This probe works in aqueous solution over a wide pH range under mild conditions and can be used for rapid; highly selective and sensitive detection of Sec with significant near-infrared fluorescent turn-on signal changes. In addition, it features a remarkable large Stokes shift (192 nm) and a low detection limit (60 nM) for Sec with a wide linear range (0-70 mu M). Moreover, this probe can be conveniently used to detect Sec in serum samples, living cells, and animals, indicating it holds great promise for biological applications.