Confirmation of Zika virus infection through hospital-based sentinel surveillance of acute febrile illness in Uganda, 2014-2017
Confirmation of Zika virus infection through hospital-based sentinel surveillance of acute febrile illness in Uganda, 2014-2017
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DOI：
10.1099/jgv.0.001113
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发表时间：
2018-09-01
影响因子：
3.8
通讯作者：
Lutwama, Julius Julian
中科院分区：
文献类型：
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作者：
Kayiwa, John Timothy;Nankya, Annet Martha;Lutwama, Julius Julian
寨卡病毒(ZIKV)由伊蚊传播，1947年首次在乌干达分离到寨卡病毒。2014年2月至2017年10月，乌干达病毒研究所与美国疾病控制和预防中心合作，在恩孔孔杰卢圣弗朗西斯医院对急性发热病(AFI)患者进行了虫媒病毒监测。对384份血清标本进行了黄热病病毒、西尼罗河病毒、登革病毒、基孔肯雅病毒和寨卡病毒免疫球蛋白抗体检测。在384份标本中，5份为ZIKV IgM阳性。在这5例中，3例经空斑减少中和试验(PRNT)证实为寨卡病毒感染。在其余两例中，一例被确定为非特异性黄病毒感染，一例经逆转录聚合酶链式反应(RT-PCR)确认为甲型病毒阳性。这项研究提供了50年来乌干达实验室确认的寨卡病毒感染的第一个证据，并强调了加强哨点监测的必要性。
Zika virus (ZIKV), transmitted by Aedes species mosquitoes, was first isolated in Uganda in 1947. From February 2014 to October 2017, the Uganda Virus Research Institute, in collaboration with the US Centers for Diseases Control and Prevention, conducted arbovirus surveillance in acute febrile illness (AFI) patients at St Francis hospital in Nkonkonjeru. Three hundred and eighty-four serum samples were collected and tested for IgM antibodies to yellow fever virus (YFV), West Nile virus (WNV), dengue virus (DENV), chikungunya virus (CHIKV) and ZIKV. Of the 384 samples, 5 were positive for ZIKV IgM. Of these five, three were confirmed by plaque reduction neutralization test (PRNT) to be ZIKV infections. Of the remaining two, one was determined to be a non-specific flavivirus infection and one was confirmed to be alphavirus-positive by reverse transcriptase polymerase chain reaction (RT-PCR). This study provides the first evidence of laboratory-confirmed ZIKV infection in Uganda in five decades, and emphasizes the need to enhance sentinel surveillance.