Measuring Antibacterial Autophagy
Measuring Antibacterial Autophagy
复制标题
DOI：
10.1007/978-1-4939-8873-0_45
复制
发表时间：
2019-01-01
期刊：
影响因子：
--
通讯作者：
Randow, Felix
中科院分区：
文献类型：
--
作者：
Boyle, Keith B.;Randow, Felix
从膜封闭的空泡逃逸到细胞的胞质溶胶的细菌被自噬靶向，自噬识别细菌并将细菌捕获到自噬体中，其中它们的增殖受到限制。在这里，我们讨论了两种方法来评估抗菌自噬：（1）可视化和计数的自噬蛋白募集到附近的胞质细菌通过免疫荧光显微镜和（2）测量自噬依赖的限制细菌增殖的菌落形成单位测定。
Bacteria that escape from membrane-enclosed vacuoles to the cytosol of cells are targeted by autophagy, which recognizes and captures bacteria into autophagosomes wherein their proliferation is restricted. Here we discuss two means by which antibacterial autophagy is assessed: (1) the visualization and enumeration of autophagy protein recruitment to the vicinity of cytosolic bacteria by means of immunofluorescence microscopy and (2) the measurement of autophagy-dependent restriction of bacterial proliferation by means of colony-forming unit assay.