Expression of a novel nuclear protein is correlated with neuronal differentiation in vivo.
Expression of a novel nuclear protein is correlated with neuronal differentiation in vivo.
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一种新型核蛋白的表达与体内神经元分化相关。
DOI：
10.1002/neu.480230304
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Hapner,SJ
中科院分区：
文献类型：
--
作者：
Paden,CM;Cranston,H;Hapner,SJ
我们报道了一种单克隆抗体（MAb 526）的生产，该抗体识别在整个大鼠神经系统神经元中表达的一种新的、受发育调节的核蛋白。全脑和细胞核提取物的SDS - PAGE和免疫印迹分析表明，MAb 526可以识别表观分子量为42 kD的单个核蛋白（np），命名为np526，以及稍大的细胞质蛋白（约44 kD）。光镜下免疫细胞化学显示np526存在于中枢和周围神经系统的所有类型的神经元中。纤维和原生质星形胶质细胞的细胞核也有免疫反应，但少突胶质细胞的细胞核呈阴性。阳性，但高度变化的免疫细胞化学染色非神经细胞核在各种其他组织中也被观察到。使用预包埋过氧化物酶方法的电镜（EM）免疫细胞化学显示，np526与神经元中的常染色质或浓缩染色质束的边缘相关，表明它可能是染色体蛋白。最有趣的是，np526的表达在大脑中受到发育调节。对胚胎日(E) 16至出生日(P) 4发育中的大脑皮层和P4至P18发育中的小脑进行免疫细胞化学分析发现，np526在有丝分裂停止后首先出现在中枢神经元中，核染色强度在随后的神经元成熟过程中增加。据我们所知，np526是第一个假定的染色体蛋白，其表达与哺乳动物神经元的早期有丝分裂后分化精确相关。
We report the production of a monoclonal antibody (MAb 526) that recognizes a novel, developmentally regulated nuclear protein expressed in neurons throughout the rat nervous system. Analysis of whole brain and cell nuclear extracts by SDS‐PAGE and immunoblotting determined that MAb 526 recognizes a single nuclear protein (np) of apparent molecular weight 42 kD, designated np526, as well as a slightly larger (ca. 44 kD) cytoplasmic protein. Light microscopic immunocytochemistry showed np526 to be present in neurons of all types throughout the central and peripheral nervous systems. Nuclei of both fibrous and protoplasmic astrocytes were also immunoreactive, but oligodendrocyte nuclei were negative. Positive, but highly variable immunocytochemical staining of nonneural cell nuclei in a variety of other tissues was also observed. Electron microscopic (EM) immunocytochemistry using pre‐embedding peroxidase methods revealed that np526 is associated with euchromatin or with the edges of condensed chromatin bundles in neurons, indicating that it is likely to be a chromosomal protein. Most interestingly, the expression of np526 was found to be developmentally regulated in brain. Immunocytochemical analysis of the developing cerebral cortex from embryonic day (E) 16 to postnatal day (P) 4 and cerebellum from P4 to P18 revealed that np526 first appears in central neurons following the cessation of mitosis and that the intensity of nuclear staining increases during subsequent neuronal maturation. To our knowledge, np526 is the first presumptive chromosomal protein whose expression has been precisely correlated with the early postmitotic differentiation of mammalian neurons.