UPHILL TRANSPORT INDUCED BY COUNTERFLOW
UPHILL TRANSPORT INDUCED BY COUNTERFLOW
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逆流引起的上坡运输
DOI：
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发表时间：
1957
期刊：
影响因子：
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通讯作者：
W. Wilbrandt
中科院分区：
文献类型：
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作者：
T. Rosenberg;W. Wilbrandt
1. 在含有对两种底物具有亲和力的移动载体的膜运输系统中，在一定条件下，相对于其中一种底物的浓度梯度能够诱导另一种底物的“上坡”运输（与浓度梯度相反）。2. 在动力学处理中，导出了这种“流致上坡输运”的定量条件及其一些特征。3. 实验证明，在人红细胞中，甘露糖或未标记葡萄糖的浓度梯度诱导标记葡萄糖的上坡运输。4. 研究表明，流动诱导的上坡运输仅是移动载体系统的特征，而在底物与固定膜组分（“吸附膜”）结合的系统中则不会出现，尽管这样的系统可能产生相同的运输动力学。两种体系的通量比也不同，吸附膜符合Ussing准则，载体膜不符合Ussing准则。5. 由此得出结论，人体红细胞中的运输系统必须包含一个移动载体，与葡萄糖和甘露糖相同。
1. In a membrane transport system containing a mobile carrier with affinities for two substrates a concentration gradient with respect to one of the substrates under certain conditions is able to induce an "uphill" transport (against the concentration gradient) of the other. 2. In a kinetic treatment quantitative conditions for such a "flow-induced uphill transport" and some of its characteristics are derived. 3. Experimentally the uphill transport of labelled glucose induced by a concentration gradient for mannose or unlabelled glucose is demonstrated in the human red cell. 4. It is shown that the flow-induced uphill transport is a feature characteristic for mobile carrier systems only and is not to be expected in systems in which the substrate is bound to a fixed membrane component ("adsorption membrane"), although such a system may yield identical transport kinetics. Also with respect to Ussing's flux ratio the two systems are different, the adsorption membrane meeting Ussing's criterion, the carrier membrane not. 5. It is concluded that the transport system in the human red cells must contain a mobile carrier, identical for glucose and mannose.