Effect of iron and carbon monoxide on fibrinogenase-like degradation of plasmatic coagulation by venoms of four Crotalus species
Effect of iron and carbon monoxide on fibrinogenase-like degradation of plasmatic coagulation by venoms of four Crotalus species
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DOI：
10.1097/mbc.0000000000000529
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发表时间：
2017-01-01
影响因子：
1.1
通讯作者：
Boyle, Patrick K.
中科院分区：
文献类型：
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作者：
Nielsen, Vance G.;Redford, Daniel T.;Boyle, Patrick K.
每年，成千上万的人遭受毒蛇咬伤，通常来自去纤维蛋白的物种。铁和一氧化碳（CO）通过调节纤维蛋白原来改善凝血动力学，如在各种蝮蛇和响尾蛇中所证明的。因此，我们试图确定用铁和CO预处理血浆是否可以减弱蛇毒介导的纤维蛋白原的催化作用，所述纤维蛋白原是从具有已知纤维蛋白原酶活性的四种常见响尾蛇物种中获得的。在暴露于北方太平洋响尾蛇、亚利桑那黑响尾蛇、草原响尾蛇和红钻响尾蛇的毒液之前，用氯化铁（0- 10 mmol/l）和CO-释放分子-2（0- 100 mmol/l）预处理人血浆。使用的毒液的浓度降低凝血功能的一个或多个动力学参数的至少50%的正常值。用血栓弹力图测定凝血动力学。三种蛇毒显著降低血浆凝血动力学，延长凝块形成的起始时间，降低凝块生长速度并降低凝块强度。然而，红钻响尾蛇毒液暴露导致混合凝血动力学，显着减少凝血发作的时间，而不降低凝块生长的速度。铁和CO衰减这些凝血动力学的变化在一个物种特异性的方式。其他纤溶毒素的进一步体外研究表明，以确定铁和CO是否可以减弱毒液受损凝血。版权所有（C）2017威科医疗集团All rights reserved.
Annually, thousands suffer poisonous snake bite, often from defibrinogenating species. Iron and carbon monoxide (CO) improve coagulation kinetics by modulation of fibrinogen as demonstrated in various Agkistrodon species and Crotalus atrox. Thus, we sought to determine whether pretreatment of plasma with iron and CO could attenuate venom-mediated catalysis of fibrinogen obtained from four common Crotalus species with known fibrinogenase activity. Human plasma was pretreated with ferric chloride (0-10mmol/l) and CO-releasingmolecule-2 (0-100mmol/l) prior to exposure to venom from a Northern Pacific rattlesnake, Arizona black rattlesnake, prairie rattlesnake, or red diamond rattlesnake. The concentration of venom used decreased coagulation function of one or more kinetic parameters by at least 50% of normal values. Coagulation kinetics were determined with thrombelastography. Three snake venoms significantly degraded plasmatic coagulation kinetics, prolonging the onset to clot formation, diminishing velocity of clot growth and decreasing clot strength. However, red diamond rattlesnake venom exposure resulted in mixed coagulation kinetics, significantly decreasing the time to onset of coagulation without decreasing the velocity of clot growth. Iron and CO attenuated these coagulation kinetic changes in a species-specific manner. Further in vitro investigation of other fibrinogenolytic venoms is indicated to determine if iron and CO can attenuate venom compromised coagulation. Copyright (C) 2017 Wolters Kluwer Health, Inc. All rights reserved.